以蛋白质芯片为中药蛋白质／肽相互作用载体分析羚羊角的生物学特征

目的对传统中药羚羊角水溶液中蛋白质，肽成分进行蛋白质组分析。方法采用饱和硫酸胺沉淀，透析脱盐冻干法获得经煎煮或冷浸的羚羊角蛋白质/肽,美国Ciphergen Biosystems Inc．ProteinChip OR Biology System（PBSⅡ＋）及配套的NP10芯片，激光解析，离子化一飞行时间质谱（SELDI -TOF -MS, Surface enhanced laser desorption/ionization time-of-flight mass spectrometry）技术．分析羚羊角水溶液中1500～13000 Da蛋白质，肽分布及其分子量。结果共计得出25个蛋白质，肽分子量峰，分子量部分差值与某些氨基酸残基分子量相近。并且在煎煮和冷浸两种方法中显示出部分蛋白质，肽分子量相近，说明可能属同一肽段。为筛选羚羊角的活性效应成分提供了有价值的线索。结论通过分析，可以形成羚羊角水提液或水煎液蛋白质／肽成分质量指纹图（PMF），并作为羚羊角数字化质控标准。为分离、纯化及验证羚羊角功能相关活性蛋白质，肽成分打下基础。

Biological characters of Chinese drug Antelope Horn analyzed with protein chip used as interactions carrier of protein/peptide

Objective To analyze the proteomics in Chinese drug water soluble protein/ peptide from Antelope horn by means. Methods The dialysis-desalination-freeze drying with satisfying sulphuric acid amine sediment were to get the proteirppeptide from Antelope horn and interval 1 500-13 000 Da protein/peptide distribution and their molecular weight in the Antelope horn solution were analyzed with Ciphergen Biosystems Ine.ProteinChipR Biology System （American PBS 11 ＋ ） and the combined NP10array, SELDI-TOF-MS, Surface enhanced laser desorption/ionization time-of-flight mass spectrometry technique. Results The 25 peaks of protein/peptide molecular weight were meaured, in which, some disparity and some amino acid residue molecular weight were approximate. In the two methods of pretreated Antelope horn,the molecular weight of some proteirdpeptide were extreme close, which seggested peptides might be deduced to the same peptides. These messages provided valuable further screening active and effective ingredient. Conclusion The finger print map peptide are gained to be the digitizated quality control standard for the fundation of abstract, purifieate and verifieate the protein/peptide in Chinese medicine. that these clues for of protein/ the further