Localization of P2X7 receptor immunoreactivity in the dorsal root ganglia of guinea pig |
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Authors: | Bai Xuegong Jiang Ling Xiang Zhenghua |
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Affiliation: | (1) Department of Neurology, Xiang fan Hospital, Tongji Medical College, Huazhong University of Science and Technology, 441021 Xiang fan, China;(2) Shanghai University of Science and Technology, 200093 Shanghai, China;(3) Department of Biochemistry and Molecular Biology, Second Military Medical University, 200433 Shanghai, China |
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Abstract: | Summary The P2X7 receptor mRNA and proteins in guinea-pig dorsal root ganglia (DRG) were studied by using RT-PCR and immunohistochemistry. The co-localization of P2X7 receptor with four cytochemical markers, the neurofilament protein NF200, S100, substance P and isolectin B4 (IB4) binding glyco-conjugates, were also examined. It was found that P2X7 receptor immunoreactivity (P2X7R-IR) was present mostly in large- and medium-sized DRG neurons (62%±9% and 36%±6% respectively in all P2X7R-IR neurons). All the P2X7R-IR neurons were also NF200 and S100 immunopositive. However, in a small number of NF200 or S100 immunopositive neurons no P2X7R-IR was detectable. All the IB4-positive or substance P-immunopositive neurons had no P2X7R-IR. These results demonstrate that P2X7 receptors are expressed in a large subpopulation of DRG neurons and they may play a role in the transduction of specific peripheral sensory signals. BAI Xuegong, male, born in 1968. Associate Professor |
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Keywords: | P2X7 receptor ATP receptor purinoreceptor dorsal root ganglion NF200 S100 isolectin B4 substance P |
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