| 基于ITS2条形码的中药材天南星及其混伪品DNA分子鉴定 |
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| 引用本文： | 石林春,陈俊,向丽,宋经元,姚辉.基于ITS2条形码的中药材天南星及其混伪品DNA分子鉴定[J].中国中药杂志,2014,39(12):2176-2179. |
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| 作者姓名： | 石林春 陈俊 向丽 宋经元 姚辉 |
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| 作者单位： | 中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193;中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193;中国中医科学院 中药研究所, 北京 100700;中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193;中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193 |
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| 基金项目： | 国家自然科学基金项目（81373922）；中国医学科学院药用植物研究所创新团队发展计划项目 |
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| 摘 要： | 该研究收集天南星及其混伪品7种，共58份样品，通过DNA提取和PCR扩增，经注释后获得其ITS2条形码，然后进行序列变异分析和NJ树聚类分析。结果表明天南星3种基原天南星、异叶天南星和东北天南星的种内K2P距离均小于其种间K2P距离，在NJ聚类树上天南星3种基原分别聚为独立的支。天南星3种基原与天南星混伪品聚为不同的支。因此，ITS2作为DNA条形码可以鉴定天南星3种基原及其混伪品，DNA条形码技术可为天南星及其混伪品的鉴定提供新的方法。
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| 关 键 词： | 天南星 ITS2 DNA条形码 鉴定 |
| 收稿时间： | 2014/3/17 0:00:00 |
DNA barcoding identification between Arisaematis Rhizoma and its adulterants based on ITS2 sequences |
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| SHI Lin-chun,CHEN Jun,XIANG Li,SONG Jing-yuan and YAO Hui.DNA barcoding identification between Arisaematis Rhizoma and its adulterants based on ITS2 sequences[J].China Journal of Chinese Materia Medica,2014,39(12):2176-2179. |
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| Authors: | SHI Lin-chun CHEN Jun XIANG Li SONG Jing-yuan and YAO Hui |
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| Institution: | Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China |
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| Abstract: | Fifty-eight samples belonging to 7 species of Arisaematis Rhizoma and its adulterants were collected. The ITS2 locus was employed as a DNA barcode and amplified, sequenced and assembled for all of the collected samples. Then, ITS2 sequences have been annotated using HMM-based method. The intra- and inter-specific variations were calculated and NJ tree was constructed using MEGA 6.0 software. The results showed that inter-specific K2P distances were significantly larger than intra-specific distances for all of the three origin species of Arisaematis Rhizoma. Furthermore, three origin species, Arisaema amurense, A. erubescens and A. heterophyllum, can be respectively formed to be a single branch with high bootstrap values. It is concluded that ITS2 can be used to correctly identify Arisaematis Rhizoma from its adulterants and the application of ITS2 in the identification of traditional Chinese medicine has an important prospective. |
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| Keywords: | Arisaematis Rhizoma ITS2 DNA barcoding identification |
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