一测多评法测定山银花提取物中6种绿原酸类成分

以绿原酸为内标，测定新绿原酸、隐绿原酸、3，4-O-双咖啡酰奎宁酸、3，5-O-双咖啡酰奎宁酸和4，5-O-双咖啡酰奎宁酸的相对校正因子，建立山银花提取物中6种绿原酸类成分的一测多评定量分析方法。通过考察不同色谱仪和不同色谱柱以验证相对校正因子的耐用性，同时采用外标法对4批山银花药材及20批山银花提取物进行分析，并将其含量测定结果与一测多评法测定结果进行比较。结果表明相对校正因子的耐用性良好，4批山银花药材及20批山银花提取物一测多评法与外标法所得结果无显著差异。所建立的含量测定方法可用于同时测定山银花提取物中6种绿原酸类成分。

Determination of six chlorogenic acids in Lonicerae Flos extract by quantitative analysis multi-components with single marker

This study is to determine six chlorogenic acids (chlorogenic acid, 5-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid) by quantitative analysis of multi-components with a single marker (QAMS). Chlorogeinc acid was used as internal reference to calculate the relative correction factors (RCF) of five compounds. Then the ruggedness of relative correction factors was tested on different instruments and columns. Meanwhile, a total of 4 batches of Lonicerae Flos and 20 batches of Lonicerae Flos extract with five different processing procedures were analyzed by external standard method (ESM) and QAMS, respectively. The ruggedness of relative correction factors was good. And the analytical results calculated by ESM and QAMS showed no difference. The quantitative method established was suitable for the quality evaluation of Lonicerae Flos extract.