天山雪莲悬浮培养细胞中紫丁香苷、绿原酸和1, 5-二咖啡酰奎尼酸的生物合成调控

紫丁香苷、绿原酸和1，5-二咖啡酰奎尼酸为野生天山雪莲中的3种活性化学成分，具多种药理活性，在药材中含量较低。研究在筛选得到天山雪莲上述3种化学成分高产细胞系的基础上，通过外源添加碳源和生物合成前体的方法调控紫丁香苷、绿原酸和1，5-二咖啡酰奎尼酸在天山雪莲培养细胞中的生物合成，极大地提高了其含量和产量。仅需培养16 d，紫丁香苷、绿原酸和1，5-二咖啡酰奎尼酸的质量浓度即可以分别达到339.0，225.3，512.7 mg·L^-1；三者的含量分别为野生药材的67.9，1.9，10.6倍。研究为最终实现通过天山雪莲细胞培养同时规模化生产以上3种活性成分打下了坚实基础，具有理论和实际应用意义。

Regulation of syringin, chlorogenic acid and 1, 5-dicaffeoylquinic acid biosynthesis in cell suspension cultures of Saussurea involucrata

Syringin, chlorogenic acid and 1,5-dicaffeoylquinic acid are three main bioactive ingredients in herbs of Saussurea involucrata with various pharmacological properties, while their contents are very low. In this study, the biosynthesis of syringin, chlorogenic acid and 1,5-dicaffeoylquinic acid in the cell suspension cultures of S. involucrata were regulated by feeding carbon sources and precursors, which resulted in a great increase of the contents and yields of the above three bioactive ingredients. After 16 days of fermentation, the yields of syringin, chlorogenic acid and 1,5-dicaffeoylquinic acid reached 339.0, 225.3, 512.7 mg·L^-1, respectively. Meanwhile, their contents increased up to 67.9, 1.9, 10.6 times of wild medicinal material, respectively. The results provided a solid basis for further studies on application of cell suspension cultures of S. involucrata for large-scale production of bioactive compounds syringin, chlorogenic acid and 1,5-dicaffeoylquinic acid.