不同氨基酸对雷公藤不定根生长及次生代谢产物含量的影响

以雷公藤不定根为材料，通过在MS培养基中添加不同浓度的天冬氨酸，异亮氨酸，半胱氨酸和精氨酸，研究其对雷公藤不定根生长和次生代谢产物含量的影响。结果表明：第3周添加0.25 mmol·L^-1天冬氨酸时，虽然不定根增长量为对照的80%，但不定根及培养液中雷公藤内酯醇含量分别为对照的1.36，1.3倍，雷公藤吉碱含量分别为对照的1.16，1.37倍，雷公藤次碱的含量分别为对照的1.22，1.63倍。第3周添加0.05 mmol·L^-1的异亮氨酸时不定根增长量为对照的97.3%，不定根及培养液中雷公藤吉碱分别为对照的1.02，1.27倍，雷公藤次碱的含量分别为对照的1.36，1.15倍。第1周添加0.25 mmol·L^-1半胱氨酸时，不定根增长量仅为对照的77.5%，但不定根中雷公藤内酯醇含量为对照的1.87倍。第2周添加1.00 mmol·L^-1半胱氨酸时，培养液中雷公藤次碱含量为对照的2.97倍。在第3周精氨酸浓度为0.5 mmol·L^-1时，不定根增长量为对照124.2%，雷公藤吉碱和次碱的含量分别为对照的1.3，1.4倍。

Effects of amino acid on growth and secondary metabolites contents of adventitious roots of Tripterygium wilfordii

The adventitious root of Tripterygium wilfordii was used as experiment material to study effects of various concentration of aspartic acid, isoleucine, cysteine and arginine in MS medium on the growth and triptolide, wilforgine, wilforine contents of the adventitious roots. The results showed that compared with the control, supplemented with 0.25 mmol·L^-1 aspartic acid at 3rd week, the growth of the adventitious roots only accounted for 80%, but the content of triptolide of the adventitious roots and the medium was 1.36, 1.30 times, the content of wilforgine was 1.16, 1.37 times, the content of wilforine was 1.22, 1.63 times, respectively. At 3rd week 0.05 mmol·L^-1 isoleucine, the growth of adventitious roots was 97.3%, wilforgine of adventitious roots and medium 1.02, 1.27 times, wilforine 1.36 times and 1.15 times. At 1st week 0.25 mmol·L^-1 cysteine, the growth of the adventitious roots comprised 77.5% of the control, while content of triptolide of adventitious roots reached 1.87 times. At 2nd week 1.00 mmol·L^-1 cysteine, the growth of adventitious roots was 44.6% of the control, the content of wilforine in medium was 2.97 times. At 3rd week 0.50 mmol·L^-1 arginine, the growth of adventitious roots was 124.2%, the content of wilforgine and wilforine was 1.3, 1.4 times, respectively.