In vivo evaluation of the dopaminergic neurotransmission system using [123I]FP‐CIT SPECT in 6‐OHDA lesioned rats |
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Authors: | Aida Niñerola‐Baizán Santiago Rojas Mercè Bonastre Raúl Tudela Francisco Lomeña Javier Pavía Concepció Marin Domènec Ros |
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Institution: | 1. Unitat de Biofísica i Bioenginyeria, Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain;2. Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina, Barcelona, Spain;3. Thrombotargets Europe S.L., Castelldefels, Spain;4. INGENIO, IRCE, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain;5. Servei de Medicina Nuclear, Hospital Clínic, Barcelona, Spain;6. Centro de Investigación Biomédica en Red de Salud Mental, Barcelona, Spain;7. Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain;8. Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas, Barcelona, Spain |
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Abstract: | The 6‐hydroxydopamine (6‐OHDA) rodent model of Parkinson's disease (PD) has been used to evaluate the nigrostriatal pathway. The aim of this work was to explore the relationship between the degree of 6‐OHDA‐induced dopaminergic degeneration and 123I]FP‐CIT binding using single photon emission computed tomography (SPECT). Fourteen rats received a 6‐OHDA injection (4 or 8 µg) into the left medial forebrain bundle. After 3 weeks, magnetic resonance imaging and scans with a small‐animal SPECT system were performed. Finally, the nigrostriatal lesion was assessed by immunohistochemical analysis. Immunohistochemical analysis confirmed two levels of dopaminergic degeneration. Lesions induced by 6‐OHDA diminished the ipsilateral 123I]FP‐CIT binding by 61 and 76%, respectively. The decrease in tracer uptake between control and lesioned animals was statistically significant, as was the difference between the two 6‐OHDA lesioned groups. Results concluded that 123I]FP‐CIT SPECT is a useful technique to discriminate the degree of dopaminergic degeneration in a rat model of PD. Copyright © 2014 John Wiley & Sons, Ltd. |
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Keywords: | [123I]FP‐CIT 6‐OHDA SPECT rat Parkinson's disease molecular imaging |
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