| HSV-2DNA疫苗诱导小鼠免疫应答研究 |
| |
| 引用本文： | 洪艳,杨连华,陈勇,经络,蒋骏航,王怡婷. HSV-2DNA疫苗诱导小鼠免疫应答研究[J]. 中国公共卫生, 2003, 19(9): 1079-1080 |
| |
| 作者姓名： | 洪艳 杨连华 陈勇 经络 蒋骏航 王怡婷 |
| |
| 作者单位： | 浙江省医学科学院生物工程所基因室,杭州,310013 |
| |
| 基金项目： | 浙江省自然科学基金 (398484)，浙江省卫生厅资助项目 |
| |
| 摘 要： | 目的 探讨含有单纯疱疹病毒Ⅱ型(HSV-2)糖蛋白D全基因序列的重组质粒作为DNA疫苗的可能性。方法 采用PCR方法获得HSV-2gD片段，构建含HSV-gD片段的重组质粒pcDNA3-gD。重组质粒作为DNA疫苗免疫小鼠，观察其对小鼠免疫效果及保护作用。结果 重组质粒pcCDNA3-gD免疫小鼠后可产生特异性抗体，可保护小鼠免受HSV-2的致死性攻击，存活率达75％。结论 重组质粒pcDNA3-gD可诱导小鼠产生特异性免疫反应，可以作为HSV的候选DNA疫苗。
|
| 关 键 词： | 单纯疱疹病毒 DNA疫苗 聚合酶链反应 糖蛋白D |
| 文章编号： | 1001-0580(2003)09-1079-02 |
| 修稿时间： | 2003-03-27 |
Immune response induced by herpes simplex virus-2 DNA vaccine in mice |
| |
| HONG Yan,YANG Lian-hua,CHEN Yong,et al.. Immune response induced by herpes simplex virus-2 DNA vaccine in mice[J]. Chinese Journal of Public Health, 2003, 19(9): 1079-1080 |
| |
| Authors: | HONG Yan YANG Lian-hua CHEN Yong et al. |
| |
| Affiliation: | HONG Yan,YANG Lian-hua,CHEN Yong,et al.Zhejiang Academy of Medical Science |
| |
| Abstract: | Objective To evaluate the protective efficacy of DNA vaccine expressing herpes simplex type 2(HSV-2) full-length glycoprotein D(gD).Methods The whole encoding sequence of gD was amplified from HSV-2 genome by PCR,and then cloned into downstream of PCMV of pcDNA3,and resulted in the HSV-2 DNA vaccince - pcDNA3-gD.pcDNA3-gD was inoculated to mice by bilateral intramuscular injection into the rear legs.Results The mice inoculated with pcDNA3-gD could produce specific antibodies and survive after the lethal challenge with HSV-2.The rate of survival rate was 75%.Conclusion pcDNA3-gD can induce specific immune response and may be a candidate DNA vaccine D. |
| |
| Keywords: | herpes simplex virus DNA vaccine polymerase chain reaction(PCR) glycoprotein D(gD) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
