雷公藤红素联合5-氟尿嘧啶在人结肠癌细胞中的相互作用 |
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引用本文: | 罗伟,陈卫昌.雷公藤红素联合5-氟尿嘧啶在人结肠癌细胞中的相互作用[J].中国航天工业医药,2008(12):4-7. |
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作者姓名: | 罗伟 陈卫昌 |
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作者单位: | 江苏省苏州大学附属第一医院消化内科,215006 |
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摘 要: | 目的体外观察雷公藤红素与5-氟尿嘧啶(5-FU)联用在人结肠癌HCT-116细胞增殖中的相互作用。方法采用MTT法观察不同浓度雷公藤红素及5-FU单独或联合应用对结肠癌细胞的生长抑制作用,并利用中效原理判断联合用药的效果。结果雷公藤红素和5-FU单独应用时,随药物浓度增加对HCT-116细胞的抑制作用也增加,中效浓度分别为3.533μmol/L,9.254μmol/L,两药联用时在大部分效应范围(0〈fa〈83%)表现协同作用(CI〈1),中效浓度为6.433μmol/L.其中雷公藤红素0.099μmol/L,5-FU6.33μmol/L。两药合用给药时间及次序不同时不同时,合用效应无明显差异。结论上述两种药物联合应用时具有较好的协同效应,且药物效应与给药顺序无关。
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关 键 词: | 雷公藤红素 5-氟尿嘧啶 中效原理 合用指数 联合 |
The interaction between celastrol and 5-fluorouracil on human colon cancer cells |
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Authors: | Luo Wei Chen Weichang |
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Institution: | .(Depanrnent of Gastroenterology, The First Hospital of Soochow University, Suzhou 215006) |
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Abstract: | Objective To study the interaction of combination of celastrol and 5-fluorouracil (5-FU) on cell proliferation of human colon cells in vitro. Methods The inhibitory effects of celastrol,5-FU and celastrol combined with 5-FU on the growth of HCT-116 cells were determined by MTF assay.The median-effects principle was used to assess the effect of the combination therapy. Results The inhibitory effects of celastrol and 5-FU on the growth of HCT-116 increased with their concentration when these two drugs were used alone,and the median-effects concentration were 3.533μmol/L,9.254μmol/L, respectively.In the majority of effect region (0〈fa〈83%),celastrol was synergistic with 5-FU (CI〈1)in HCT-116 cells,and the median-effects concentration was 6.433μmol/L (celastrol 0.099μmol/L ,5-FU 6.33μmol/L) .The sequence of administration did not influnence the cytotoxic activity of the combined antitumor drugs. Conclusion There is a good synergistic effect when celastrol and 5-FU are used together.The sequence of administration aren't factors influencing the killing effect. |
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Keywords: | Celastrol 5-fluorouracil (5-FU) Median-effect principle Conbination index(CI) Combined |
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