The Ca^2＋ Over Loading Effect of Oxidized Low Density Lipoprotein on Macrophages

Purpose The Ca^2＋ over loading effect of oxidized low density lipoprotein （OLDL） on macrophages and its mechanisms were explored in these studies. Methods C57BL/6J mouse peritoneal macrophages were incubated in 10mg/L oxidized low density lipoprotein （OLDL）, the intracellular Ca^2＋ level was determined with the technique of Ca^2＋ fluorescent indicator, and the membranous Ca^2＋-ATPase activity was determined with the assay of NADH-oxidizing coupling spectrum-alteration. Results The intracellular Ca^2＋ level was 2.7 times higher than the control, and the membranous Ca^2＋-ATPase activity was 24.0% of the control. Conclusion OLDL exerted a Ca^2＋ over loading effect on macrophages, and this was probably related to the opening of the membranous Ca^2＋ channels and the inactivating of the membranous Ca^2＋-ATPase.