| 脐血浆神经营养活性蛋白成分对CD34+细胞增殖分化的影响 |
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| 引用本文： | 张丽,李倩如,杜英,杨波,李国喜,胡祥.脐血浆神经营养活性蛋白成分对CD34+细胞增殖分化的影响[J].中国神经再生研究,2011,15(6):995-999. |
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| 作者姓名： | 张丽 李倩如 杜英 杨波 李国喜 胡祥 |
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| 作者单位： | 河南省红十字血液中心，河南省郑州市 450053；郑州大学基础医学院微生物学与免疫学教研室，河南省郑州市450001,郑州大学基础医学院微生物学与免疫学教研室，河南省郑州市450001,郑州大学基础医学院微生物学与免疫学教研室，河南省郑州市450001,郑州大学第一临床医院神经外科，河南省郑州市 450052,深圳市北科细胞工程研究所，广东省深圳市 518000,深圳市北科细胞工程研究所，广东省深圳市 518000 |
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| 摘 要： | 背景：迄今为止，人们对于脐血浆中是否存在活性成分及其对于神经发育及神经损伤修复的作用认识尚不足，有待深入研究。
目的：检测脐血浆生物活性成分，观察其在神经发育及神经损伤修复中的作用。
方法：采用抗体芯片技术对脐血浆和健康青年女性静脉血浆活性成分进行对比分析，采用免疫磁珠从脐血中分选出CD34+细胞，经体外培养计数观察CD34+细胞增殖能力，通过免疫组化法观察细胞分化情况。
结果与结论：脐血浆中有31种蛋白分子的含量显著高于静脉血浆，其中具有促进神经再生和神经修复潜能的活性蛋白10种，包括FGF4、Frizzled-3、IL-3、RAGE、CRIM-1、Neuritin、Neuropilin-2、Neurturin、SFRP-3、Tomoregulin-1；在CD34+细胞培养液中加入脐血浆能显著促进细胞增殖，促进细胞向神经细胞分化。
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| 关 键 词： | 脐血浆 CD34+细胞 神经营养因子 FGF4 Frizzled-3 Neutrin |
Effects of neurotrophic factors in umbilical cord blood plasma on CD34+ cell proliferation and differentiation |
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| Zhang Li,Li Qian-ru,Du Ying,Yang Bo,Li Guo-xi and Hu Xiang.Effects of neurotrophic factors in umbilical cord blood plasma on CD34+ cell proliferation and differentiation[J].Neural Regeneration Research,2011,15(6):995-999. |
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| Authors: | Zhang Li Li Qian-ru Du Ying Yang Bo Li Guo-xi and Hu Xiang |
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| Institution: | Henan Province Red Cross Blood Center, Zhengzhou 450053, Henan Province, China; Department of Microbiology and Immunology, Basic Medical College, Zhengzhou University, Zhengzhou 450001, Henan Province, China,Department of Microbiology and Immunology, Basic Medical College, Zhengzhou University, Zhengzhou 450001, Henan Province, China,Department of Microbiology and Immunology, Basic Medical College, Zhengzhou University, Zhengzhou 450001, Henan Province, China,Department of Neurosurgery, First Clinical Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China,Shenzhen Beike Cell Engineering Institute, Shenzhen 518000, Guangdong Province, China,Shenzhen Beike Cell Engineering Institute, Shenzhen 518000, Guangdong Province, China |
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| Abstract: | BACKGROUND: Further studies are needed to understand if the neurotrophic factors in human umbilical cord blood plasma have the promoting effect on neural regeneration and repair.
OBJECTIVE: To investigate the component of neurotrophic factors in umbilical cord blood plasma, observe its application in neural regeneration and repair.
METHODS: The difference was compared between the human umbilical cord blood plasma (HUCBP) and peripheral venous blood plasma (PVBP) from healthy voluntary womenfolk using human antibody array. The CD34+ cells were sorted from human umbilical cord blood using immunomagnetic beads. Following cultivating in vitro and counting, proliferative ability of CD34+ cells was observed. Cell differentiation was observed using immunohistochemical method.
RESULTS AND CONCLUSION: The concentrations of 31 kinds of protein molecules were significantly higher in HUCBP than PVBP. Of them, 10 kinds of molecules thereinto have significant potential of nerve regeneration and plasticity, including FGF4, Frizzled-3, IL-3, RAGE, CRIM-1, Neuritin, Neuropilin-2, Neurturin, SFRP-3 and Tomoregulin-1. HUCBP may promote the CD34+ cells proliferation in cell culture medium and differentiation into nerve cells. |
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| Keywords: | AAA |
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