三七总皂苷对脑缺血大鼠神经血管单元的影响

目的观察三七总皂苷对脑缺血再灌注后大鼠神经血管单元(NVU)的影响,进一步探讨三七总皂苷在脑缺血中作用特点。方法 Wistar大鼠60只随机分为对照组(20只),假手术组(20只)和三七总皂苷组(20只),每个组又按再灌注后24h、7d分为2个亚组,每个亚组大鼠10只。制作右大脑中动脉阻断(MCAO)再灌注模型后,三七总皂苷组大鼠立即腹腔注射三七总皂苷,以后每日1次直至相应时间点,对照组在相同时间内腹腔注射同体积的生理盐水。采用Longa法进行神经功能缺损评分,免疫组织化学染色法检测层黏连蛋白(Laminin)、胶质纤维酸性蛋白(GFAP)、神经元核抗原(NeuN)蛋白表达。结果再灌注后24h,三七总皂苷组和对照组大鼠神经功能缺损均比术后2h加重,两组比较,差异无统计学意义(P>0.05)。再灌注后7d,三七总皂苷组和对照组大鼠神经功能缺损评分均有改善,但三七总皂苷组大鼠神经功能缺损评分改善比对照组明显,差异有统计学意义(P<0.05)。再灌注后24h,三七总皂苷组和对照组均有NeuN、GFAP和Laminin蛋白表达,三七总皂苷组比对照组增多,但两组比较差异无统计学意义(P>0.05)。再灌注后7d,对照组NeuN、GFAP和Laminin蛋白的表达增多,三七总皂苷组明显增多,与对照组比较,差异有统计学意义(P<0.05或P<0.01);假手术组NeuN、GFAP和Laminin蛋白表达无明显变化。结论三七总皂苷促进脑缺血后NVU组分神经元、胶质细胞和微血管的修复,在脑梗死中具有多靶点、多方位作用特点。

Effects of Panax Notoginseng Saponins on neurovascular unit in rats with brain ischemia

Objective To observe the effects of Panax Notoginseng Saponins （PNS） on neurovascular unit （NVU） in rats with ischemia--reperfusion and to further study the role that PNS plays in treatment of cerebral ischemia. Methods Sixty Wistar rats were randomized to a control group （ n = 20）, a sham--operation group （ n =20） and a PNS group （ n =20）. Each group was subdivided into two subgroups according to time points of 24 h and 7 d after reperfusion. After the right middle cerebral artery occlusion （MCAO）--reperfusion models were developed, rats of PNS group were given intra--peritoneal injection of PNS immediately, after- wards, once daily till the corresponding time points. Rats of control group received intra--peritoneal injection of the same volume normal saline at the same time. A Longa neurological deficit score was used to evaluate the neurological deficit grade. An immunohistochemistry staining method was used to measure the expressions of Laminin, glial fibrillary acidic protein （GFAP） and neuronal nuclei （NeuN）. Results At 24h after reperfu- sion, rats neurological deficit in PNS group and control group were more serious than those at 2 h after opera- tion, compared PNS group to control group, there was no statistically significant difference （ P 〉0.05）. At 7 d after reperfusion, neurological deficit scores in the PNS group and control group had been improved, the PNS group was superior to the control group, there was statistically significant difference （ P 〈0.05）. At 24 h after reperfusion, both PNS group and control group had positive--expressions of NeuN, GFAP and Laminin, and PNS group had more positive--expressions of NeuN, GFAP and Laminin than control group, but compared PNS group to control group, there were no statistically significant differences （ P〉0.05）. At 7 d after reper- fusion, the positive--expressions of NeuN, GFAP and Laminin in the control group elevated, the positive--ex- pressions of NeuN, GFAP and Laminin in the PNS group elevated more obviously, and compared PNS group to control group, there were statistically significant differences （ P 〈0.05 or P 〈0.01）. The positive--expres- sions of NeuN, GFAP and Laminin in the sham--operation group remained the same. Conclusion PNS canenhance the repair of NVU neurons, glial cells and microvessels in cerebral ischemia, which has the character istics of multi--target and --location function in treatment of brain infarction.