Detection of extended broad-spectrum beta-lactamases inEnterobacteriaceae in four French hospitals |
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| Authors: | P Legrand G Fournier A Buré V Jarlier M H Nicolas D Decré J Duval A Philippon |
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| Institution: | (1) Service de Bactériologie, Hôpital Henri Mondor, 51 avenue du Maréchal de Lattre de Tassigny, 94010 Créteil Cedex, France;(2) Service de Bactériologie, Hôpital Cochin, 75014 Paris, France;(3) Service de Bactériologie, Hôpital Claude Bernard, 75019 Paris, France;(4) Service de Bactériologie, Hôpital Pitié-Salpêtrière, 75651 Paris, France |
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| Abstract: | In 210 strains ofEnterobacteriaceae which were isolated in four hospitals and which showed reduced susceptibility to cefotaxime, high synergy was demonstrated between amoxicillin (20µg) + clavulanate (10µg) and cefotaxime (30µg) using a simple double-disk test. Isoelectric focusing on gel and specific iodometric detection using ceftriaxone identified four extended broad-spectrum -lactamases (isoelectric points 7.6, 6.3, 7.0 and 5.9) produced by the strains. |
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