MicroRNA-199a-3p在肾癌中的表达及作用 |
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引用本文: | 陶良俊,秦超,曹强,钱健,蔡宏宙,朱建,李普,邵鹏飞,孟小鑫,殷长军.MicroRNA-199a-3p在肾癌中的表达及作用[J].现代泌尿生殖肿瘤杂志,2012,4(4):229-232,241. |
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作者姓名: | 陶良俊 秦超 曹强 钱健 蔡宏宙 朱建 李普 邵鹏飞 孟小鑫 殷长军 |
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作者单位: | 南京医科大学第一附属医院泌尿外科,210029 |
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摘 要: | 目的 检测microRNA-199a-3p(miR-199a-3p)在肾癌细胞株和组织中的表达情况并探究miR-199a-3p在肾癌细胞中的作用.方法 利用实时定量RT-PCR检测miR-199a-3p在肾癌细胞和组织中的表达水平;利用miR-199a-3p模拟物转染肾癌细胞786-0上调miR-199a-3p后,通过CCK-8、克隆形成、Transwell以及细胞周期检测来探究其在肾癌细胞中的作用.结果 miR-199a-3p在肾癌细胞中明显低表达,在78%(14/18)的肾癌组织中亦明显低表达;上调miR-199a-3p可显著抑制肾癌细胞的增殖、存活和侵袭并能诱导细胞周期G1期阻滞.结论 我们的研究显示在肾癌中miR-199a-3p明显低表达并参与肾癌的发生、发展,这表明miR-199a-3p具有作为肾癌诊断和治疗靶点的潜能.
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关 键 词: | MicroRNA-199a-3p 抑癌基因 肾细胞癌 |
The expression and significance of microRNA-199a-3p in renal cell carcinoma |
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Authors: | TAO Liang-jun QIN Chao CAO Qiang QIAN Jian CAI Hong-yu ZHU Jian LI Pu SHAO Peng-fei MENG Xiao-xin YIN Chang-jun |
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Institution: | . Department of Urology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China |
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Abstract: | Objective To study the expression of microRNA-199a-3p (miR-199a-3p) in renal cell carcinoma (RCC) cell lines and tissues, and assess the impact of miR-199a-3p on the tumorigenesis of RCC. Methods miR-199a-3p levels in RCC cells and tissues were assessed by real-time qRT-PCR. The RCC cell line 786-0 was transfected with miR-199a-3p mimics to restore expression of miR-199a-3p. The effects of miR-199a-3p were then assessed by CCK-8, cell cycle, Transwell, and colony formation assay. Results We found that miR-199a-3p was remarkably downregulated in RCC cells and tissues. Restoration of miR-199a-3p dramatically suppressed RCC cells growth, clonability, invasion and induced G1-phase cell cycle arrest in vitro. Conclusions Collectively, these results demonstrated that miR-199a-3p was dramatically downregulated in RCC and involved in the etiology of RCC, which suggested that miR-199a-3p may offer an attractive new target for diagnostic and therapeutic intervention in RCC. |
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Keywords: | MicroRNA-199a-3p Tumor suppressor genes Renal cell carcinoma |
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