Multicopy SUP35 gene induces de-novo appearance of psi-like factors in the yeast Saccharomyces cerevisiae |
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Authors: | Yury O Chernoff Irina L Derkach Sergey G Inge-Vechtomov |
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Institution: | (1) Department of Genetics, Saint-Petersburg University, Universitetskaya 7/9, 199034 Saint-Petersburg, Russia;(2) Present address: Laboratory for Molecular Biology (M/C 067), Department of Biological Sciences, University of Illinois at Chicago, PO Box 4348, 60680 Chicago, IL, USA |
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Abstract: | Previously, we have shown that plasmid-mediated multiplication of Saccharomyces cerevisiae wild-type SUP35 gene leads to omnipotent suppression and is incompatible with psi-factor, which is an endogenous extrachromosomal suppressor. Here, we describe a frequent de-novo appearance of psi-like factors in mitotic progeny of yeast transformants containing multicopy SUP35 gene. |
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Keywords: | Mistranslation Omnipotent suppression Plasmid Extrachromosomal element |
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