|
|||||
|
|
| 氢氧化钙对人牙髓细胞内游离钙的影响 | |
| 引用本文： | 吴海珍,文玲英,史俊南,孙叶方,王春梅,杨晓峰. 氢氧化钙对人牙髓细胞内游离钙的影响[J]. 中华口腔医学杂志, 2001, 36(2): 128-129 |
| 作者姓名： | 吴海珍 文玲英 史俊南 孙叶方 王春梅 杨晓峰 |
| 作者单位： | 1. 解放军第105医院口腔科 2. 第四军医大学口腔医学院牙体牙髓科 3. 230031 合肥，解放军第105医院电镜室 4. 中国科技大学生命科学院免疫学教研室 |
| 摘 要： | 目的 检测Ca(OH)2对体外培养的人牙髓细胞内Ca^2 的影响。方法 在RPMI1640培养液中加入Ca(OH)2、CaC12和NaHCO4，培养第6代人牙髓细胞，用Flou-3荧光探针负载后，再次用Ca(OH)2刺激，激光扫描共聚焦显微镜检测细胞内Ca^2 。结果 含Ca(OH)2培养液培养的牙髓细胞，再用Ca(OH）2刺激时胞内Ca^2 升高；其他条件培养的细胞，Ca(OH2)刺激时Ca^2 无变化。结论 Ca(OH)2可导致人牙髓细胞内Ca^2 升高。 |
| 关 键 词： | 牙髓 共焦显微镜检查 氢氧化钙 细胞内游离钙 |
| 修稿时间： | 2000-02-13 |
Effect of calcium hydroxide on human pulp cell intracellular calcium ion in vitro |
|
| WU Haizhen ,WEN Lingying,SHI Junnan,et al. Effect of calcium hydroxide on human pulp cell intracellular calcium ion in vitro[J]. Chinese journal of stomatology, 2001, 36(2): 128-129 | |
| Authors: | WU Haizhen WEN Lingying SHI Junnan et al |
| Affiliation: | Department of Stomatology, 105th PLA Hospital, Hefei 230031, China. |
| Abstract: | OBJECTIVE: To investigate the influence of calcium hydroxide to intracellular calcium ion of human pulp cells in vitro. METHODS: The sixth generation of human pulp cells were maintained in RPMI 1640 medium supplemented with Fluo-3 probe and Ca (OH)2 for restimulation. The intracellular calcium ion concentration was detected by laser confocal microscope. RESULTS: The intracellular calcium ion level in Ca (OH)2 pretreatment group increased after Ca (OH)2 stimulation, while it didn't response to Ca (OH)2 stimulus in group without Ca (OH)2, CaCl2 or NaHCO4 pretreatment. CONCLUSION: It is important to pretreat the pulp cells with Ca(OH)2 in order to increase the intracellular calcium. |
| Keywords: | Dental pulp Microscopy confocal Calcium hydroxide |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |