| Toll-like receptor 2及其信号传导通路在阿片类物质诱导细胞凋亡中的作用 |
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| 引用本文： | 李艺,彭英. Toll-like receptor 2及其信号传导通路在阿片类物质诱导细胞凋亡中的作用[J]. 重庆医科大学学报, 2010, 35(9) |
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| 作者姓名： | 李艺 彭英 |
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| 作者单位： | 中山大学附属第二医院神经内科,广州,510120;中山大学附属第二医院神经内科,广州,510120 |
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| 摘 要： | 目的:成瘾物质(Addictivedrugs)导致的中毒性脑病的中枢神经系统损害以神经元凋亡为突出表现,具体机制仍有待进一步的研究探讨.方法:Toll-like receptor2(TLR2)不仅是激活机体自身免疫防御和炎症反应的重要受体,而且广泛参与多种细胞功能,包括细胞凋亡.我们使用体外培养的HEK293和高表达TLR2的HEK293 2种细胞,以及体外培养小鼠皮层原代神经元细胞,研究经过吗啡处理后细胞存活率和凋亡的变化,以探讨TLR2信号通路在吗啡诱导的细胞凋亡中的作用.结果:TLR2高表达导致吗啡诱导的细胞存活率下降和细胞凋亡的显著增加.使用MyD88抑制体竞争性抑制MyD88并阻断TLR2信号通路后,吗啡诱导的TLR2高表达细胞的凋亡也被明显抑制.长期的吗啡处理导致正常神经元的TLR2在信使RNA(mRNA)水平和蛋白水平表达明显增高,而且在TLR2功能缺失的原代神经元细胞中,吗啡诱导的caspase-3活性增高被阻断,吗啡诱导的神经元凋亡也被明显抑制.结论:TLR2信号通路参与吗啡诱导的神经元凋亡.
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| 关 键 词： | 中毒性脑病 Toll-like receptor2 吗啡 凋亡 |
The role of Toll-like receptor 2 and down streaming signaling in opioids-mediated apoptosis |
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| LI Yi,et al. The role of Toll-like receptor 2 and down streaming signaling in opioids-mediated apoptosis[J]. Journal of Chongqing Medical University, 2010, 35(9) |
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| Authors: | LI Yi et al |
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| Affiliation: | LI Yi,et al(Department of Neurology,the Second Affiliated Hospital,Sun Yat-sen University) |
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| Abstract: | Objective:Toxicenc ephalopathy caused by addictive substances,during which the neuron apoptosis was recognized as the outstanding performance in central nervous system damage,however the specific mechanisms still need further study.Methods:Toll-like receptor 2(TLR2)is not only activate the body's own immune defense and the important receptors in inflammation,but also be broad participation in a variety of cell functions,including apoptosis.The three kinds cells,HEK293 cultured in vitro,HEK293 which highly e... |
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| Keywords: | Toll-like receptor2 |
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