油酸型急性呼吸窘迫综合征血清及肺组织促炎症细胞因子变化及山莨菪碱干预的实验研究

目的:观察大鼠油酸型急性呼吸窘迫综合征(ARDS)不同时间血清及肺组织中白细胞介素-6(IL-6)、IL-8和肿瘤坏死因子(TNF-α)的水平变化以及山莨菪碱(654-2)对其影响。方法:经舌下静脉注射油酸复制大鼠ARDS模型,用酶联免疫吸附试验测定不同时间血清及肺组织匀浆上清液中IL-6、IL-8和TNF-α水平。结果:注射油酸4h组血清及肺组织中IL-6、IL-8和TNF-α水平明显高于对照组;注射油酸8h组上述细胞因子均明显低于4h组,但血清中IL-6、TNF-α和肺组织中IL-6仍高于对照组水平;注射油酸16h组血清IL-6、TNF-α水平明显低于8h组,但TNF-α水平仍高于对照组,肺组织中IL-6水平亦高于对照组。654-2干预组血清及肺组织中IL-6、TNF-α水平均明显低于未干预组。结论:IL-6、IL-8和TNF-α在大鼠油酸型ARDS炎症过程中可能起重要作用;654-2可能通过抑制IL-6、TNF-α过量分泌,减轻肺损伤,具有防治ARDS作用。

Changes of pro-inflammatory cytokines in serum and lung of rats with oleic acid-induced acute respiratory distress syndrome and the effect of anisodamine

AIM: To observe the changes of interleukin-6(IL-6), IL-8 and tumor necrosis factor-α(TNF-α) in serum and lung at different time, and the effects of anisodamine (654-2) treatment in rats with oleic acid-induced ARDS. METHODS: The ARDS model induced by intravenous injection of oleic acid in the rat was used and levels of IL-6, IL-8, TNF-α in serum and lung tissue supernatant were measured using enzyme linked immunosorbent assay (ELISA). RESULTS: Levels of serum and lung tissue IL-6, IL-8, TNF-α in oleic acid type ARDS 4 h group were increased significantly. These cytokines in oleic acid type ARDS 8 h group were lower than that of ARDS 4 h group, but serum IL-6, TNF-α and lung tissue IL-6 were still higher than that of control group . In oleic acid type ARDS 16 h group, serum IL-6, TNF-α were lower than that of the ARDS 8 h group and serum TNF-α and lung tissue IL-6 were higher than that of control group. After 654-2 treatment, the levels of serum and lung tissue IL-6, TNF-α were decreased significantly. CONCLUSION: IL-6, IL-8 and TNF- α might play important roles in the oleic acid-induced ARDS in the rat. 654-2 might alleviate ARDS by inhibiting excess production of IL-6 and TNF-α.