中链脂肪酸对THP-1巨噬细胞源性泡沫细胞胆固醇流出的影响

目的 观察中链脂肪酸对THP-1巨噬细胞源性泡沫细胞胆固醇流出的影响.方法 采用WST-1试剂检测200μmmol/L浓度的不同脂肪酸：辛酸（C8∶0）、癸酸（C10∶0）、豆蔻酸（C14∶0）、棕榈酸（C16∶0）、硬脂酸（C18∶0）、油酸（C18∶1）、亚油酸（C18∶2）、α-亚麻酸（C18∶3）对THP-1细胞活性的影响;建立ApoA1介导的胆固醇流出细胞模型;不同浓度脂肪酸（0μmmol/L、100μmmol/L、200μmmol/L）干预富集3H-胆固醇的THP-1巨噬细胞源性泡沫细胞24h后,用液体闪烁计数仪检测并计算胆固醇的流出率.结果 不同脂肪酸在200μmmol/L浓度时,对THP-1细胞无细胞毒性作用（P＞0.05）;与空白组比较,ApoA1介导的细胞胆固醇流出明显增加（P＜0.05）;随着脂肪酸浓度的增加,C8∶0、C18∶0、C18∶1和C18∶3脂肪酸对细胞胆固醇流出影响差异有统计学意义（P＜0.05）;在200μmmol/L浓度时,C8∶0和C18∶3脂肪酸与对照组比较更能促进细胞胆固醇的流出（P＜0.05）,且C8∶0脂肪酸对促进胆固醇流出的影响明显高于C10∶0、C14∶0、C16∶0、C18∶0、C18∶1、C18∶2脂肪酸（P＜0.05）.结论 中链脂肪酸C8∶0能够促进THP-1巨噬细胞源性泡沫细胞胆固醇的流出,且在200μmmol/L浓度时明显优于其他脂肪酸.

Effects of medium-chain fatty acids on cholesterol efflux in THP-1 macrophages-derived foam cells

Objective To observe the effect of medium-chain fatty acids on cholesterol efflux in THP-1 macrophages-derived foam cells. Methods WST-1 were used to detect the effect of different fatty acids （in the presence of 200 Ix mmol/L） on the cell viability of THP-1, including caprylic acid （C8 ： 0）, capric acid （C10 ： 0）, myristic Acid （C14 ： 0）, palmitic acid （C16 ： 0）, stearic acid （C18 ： 0）, oleic acid （C18 ： 1）, linoleic acid （C18 ： 2） and alpha linolenic acid （C18 ： 3）. The cell model of cholesterol efflux that mediated by ApoA1 was established. Different fatty acids （0 μmmol/L, 100μmmol/L and 200 ~ mmol/L） were used to intervene the THP-1 macrophages-derived foam cells of enriched 3H-cholesterol, then after 24 hours, the rate of cholesterol efflux were determined by the liquid scintillation. Results In the presence of 200μmmol/L, different fatty acids showed no adverse effect on the viability of THP-1 cell line （P 〉 0.05）. Compared with the blank group, ApoA1 mediated cholesterol efflux was significantly higher （P 〈 0.05）. With the increase of concentration of fatty acids, C8 ： 0, C18 ： 0, C18 ： 2 and C18 ： 3 showed significant effects on cholesterol efflux （P 〈 0.05）. In the concentration of 200 μmmol/L, C8 ： 0 and C18 ： 3 fatty acids significantly promoted cholesterol efflux comparing with control group （P 〈 0.05）, furthermore, C8 ： 0 fatty acid promoted cholesterol efflux was significantly higher than in C10 ： 0, C14 ： 0, C16 ： 0, C18 ： 0, C18 ： 1 and C18 ： 2 fatty acids groups （P 〈 0.05）. Conclusion The medium-chain fatty acid C8 ： 0 can increase cholesterol efflux in THP-1 macrophages-derived foam cells and is obviously higher than that of other fatty acids when its concentration is 200 μmmol/L.