汉防己甲素对白血病耐药细胞株K562/A02核因子κB表达的影响

目的：通过研究汉防己甲素（tetrandrine，Tet）对白血病细胞株K562及其耐药细胞株K562／A02核因子kB（nuclear factor-kaPPaB，NF-kB）表达的影响，探讨Tet逆转多药耐药的作用机制。方法：采用免疫细胞化学及蛋白印迹法分别检测1umol／L Tet作用K562和K562／A02细胞6h和12h舌，细胞NF-kB核转位水平和胞核NF—kB蛋白表达的变化。结果：Tet作用6h和12h后，对K562细胞的NF-kB核转位水平及胞核NF—kB蛋白表达均无明显影响（P〉0．05）；K562／A02细胞NF—kB核转位水平及胞核NF—kB蛋白表达明显高于K562细胞对照组（P〈0．01）；Tet作用6h和12h后，可明显降低K562／A02细胞NF-kB蛋白核转位（P〈0．05）和胞核NF—kB蛋白表达水平（P〈0．01）。作用12h较作用6h效果更显著（P〈0．05）。结论：1umol／L Tet可能通过抑制NF—kB活化逆转K562／A02细胞多药耐药性。

Effects of tetrandrine on nuclear factor-kappaB expression in leukemia multidrug-resistant cell line K562/A02

Objective: To investigate the effects of tetrandrine (Tet) on nuclear factor kappaB (NF-kappaB) expression in leukemia cell line K562 and multidrug-resistant K562/A02 cell line. Methods: The activations of NF-kappaB in K562 and K562/A02 cells and the effects of 1 mumol/L Tet on NF-kappaB expression were determined by immunocytochemistry and Western blot assay.dz Results: Tet had no effect on NF-kappaB expression in K562 cells after 6- and 12-hour treatment (P>0.05), and K562/A02 cells displayed higher level of NF-kappaB protein expression than their parental K562 cells (P<0.01). Tet could significantly down-regulate NF-kappaB protein expression and nuclear translocation in K562/A02 cells shown by immunocytochemistry and Western blot, and this decrease became more significant after 12-hour treatment than after at 6-hour treatment (P<0.05). dz Conclusion: Activation of NF-kappaB may be related to multidrug resistance of K562/A02 cell line. And the inhibition of NF-kappaB activation by Tet leads to multidrug resistance reversal in K562/A02 cell line.