乌司他丁对缺血再灌注损伤大鼠肠黏膜免疫功能的影响

目的：探讨乌司他丁（ulinastafin，UTI）对缺血再灌注（IR）损伤大鼠肠黏膜免疫屏障的保护作用及机制。方法：采用大鼠小肠缺血45min再灌注模型，治疗组于再灌注5min内尾静脉缓慢注射UTI。于再灌注后2h用免疫放射法测定血清、肠黏膜组织中分泌型免疫球蛋白A（sIgA）的含量；四甲基偶氮唑盐微量酶反应比色法（MTr）测定Peyer＇s淋巴结（pp）、上皮内淋巴细胞（IEL）和固有层淋巴细胞（LPL）增殖活力。结果：肠IR后血清和肠黏膜中sIgA含量下降，淋巴细胞增殖活性下降（P〈0．01）；不同剂量的UTI治疗组均能逆转IR后sIgA含量下降和淋巴细胞增殖活性下降，差异有显著意义（P〈0．01），而不同剂量UTI治疗组间各项指标也有显著差异（P〈0．05）。结论：乌司他丁（UTI）可参与调节肠道相关淋巴细胞免疫功能，对缺血再灌注损伤肠道免疫屏障具有保护作用。

Effect of ulinastatin on gut mucosal immune function after intestine ischemic reperfusion injury in rats

Objective:To investigate the effect of ulinastatin on gut mucosal immune function after intestine ischemic reperfusion(IR) injury in rats and its mechanism.Methods: An ischemic reperfusion rat model was used.(Ulinastatin) was injected into rat's caudal vein within 5 min after IR in treatment group.Two hours after IR,the(levels) of sIgA in plasma and intestinal mucosal were measured by radioimmunoassay.The lymphocytic proliferation(activity) in gut-associated lymphoid tissue was detected by MTT colorimetric assays.Results: Compared with sham IR group,the level of sIgA in plasma and intestinal mucosal decreased significantly,the lymphocytic proliferation activity in gut-associated lymphoid tissue was depressed remarkably after IR(P <0.01).Compared with IR group,the level of sIgA in plasma and intestinal mucosal increased and the lymphocytic proliferation activity also increased in low-dose UTI and high-dose UTI groups(P <0.01).There was a significant difference between low-dose UTI and high-dose UTI groups(P <0.05).Conclusion: Ulinastatin can improve gut mucosal(immune) function and can protect intestinal mucosal(immune) barrier from ischemic reperfusion injury.