Isolation and characterization of the mouse homolog of the preprodynorphin (Pdyn) gene |
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Authors: | Sharifi N Ament M Brennan M B Hochgeschwender U |
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Affiliation: | Unit on Molecular Genetics, NSB, Bethesda, MD, 20892, USA. |
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Abstract: | We have isolated and sequenced the mouse preprodynorphin gene (Pdyn). The Pdyn gene can encode for six biologically active dynorphin peptides. The predicted mouse preprodynorphin has 90%, 67%, and 66% identity with the predicted rat, porcine, and human preprodynorphins, respectively. Using an RT-PCR technique, we show that the Pdyn gene starts being expressed at embryonic day 12.5, with a steep increase of expression by embryonic day 14.5; in the adult mouse it is expressed in the brain, but not in liver, heart, spleen, or kidney. |
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