生存素反义寡核苷酸对血管瘤血管内皮细胞增殖及Caspase-3活性影响研究

目的 观察生存素(Survivin)反义寡核苷酸(ASODN)对人血管瘤血管内皮细胞凋亡的影响,并初步探讨其作用机制.方法 实验于2007年12月至2010年2月完成.以靶向Survivin基因中与Caspase-3结合的部位为模板,设计合成ASODN并通过脂质体将其转染至人血管瘤血管内皮细胞中.MTT观察Surv...

Effect of Survivin ASODN on proliferation of vascular endothelial cells of hemangioma and Caspase-3 activity

Objective To observe the effect of Survivin antisense oligodeoxynucleotid （ASODN） on the apoptosis of vascular endothelial cells of haemangioma and to explore its mechanism. Methods Targeting Survivin gene in Caspase-3 binding sites to design and synthesis of ASODN, different concentrations of Survivin ASODN and control sequence （scrambled ODN） were transferred into the vascular endothelial ceils of haemangioma by alipofectin reagent. The Thiazolyl blue（MTT）assay was used to measure the growth inhibitory rate. Activity of Caspase-3 enzyme was analyzed by spectrophotometry. Results After 44 hours of Survivin ASODN treatment, distinct morphologic changes characteristic of cell apoptosis were significant. The Caspase-3 activity in vascular endothelial cells of haemangioma treated with Survivin ASODN increased significantly. Conclusion Survivin ASODN can inhibit proliferation, induce apoptosis of vascular endothelial cells of haemangioma;the mechanism may be related to activating Caspase-3.