| 云南猕猴mtDNA控制区全序列测定 |
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| 引用本文： | 禹文海,黄芬,杨凤梅,鲁帅尧,赵远,沈冬,王俊斌,陈丽雄,和占龙.云南猕猴mtDNA控制区全序列测定[J].中国比较医学杂志,2011,21(12):42-45. |
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| 作者姓名： | 禹文海 黄芬 杨凤梅 鲁帅尧 赵远 沈冬 王俊斌 陈丽雄 和占龙 |
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| 作者单位： | 1. 中国医学科学院北京协和医学院医学生物学研究所,云南昆明,650118
2. 昆明理工大学生命科学与技术学院,云南昆明,650224 |
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| 基金项目： | 云南省应用基础研究面上项目，云南省技术创新人才培养基金 |
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| 摘 要： | 目的测定云南猕猴线粒体DNA控制区全序列,对其进行鉴定及进化分析。方法利用PCR技术扩增猕猴线粒体DNA控制区全序列,结合GenBank中下载的猕猴参考序列(AY612638),采用多个生物学软件对序列碱基组成、同源性、转换/颠换比等遗传信息进行分析,并基于邻接法(NJ)和最小进化法(ME)构建系统进化树。结果云南猕猴线粒体DNA控制区全长为(1 084～1 089)bp,A、T、G和C四种碱基平均含量分别为29.9%、26.9%、12.3%和30.9%,A+T含量(56.8%)高于G+C含量(43.2%)。所分析序列间的同源性为91.5%～99.5%,平均核苷酸变异率为4.5%,变异类型包括转换、颠换、插入和缺失4种形式,转换/颠换比值平均为26.1。进化树显示云南猕猴存在两个平行进化的姐妹分支。结论本研究获得了云南猕猴mtDNA控制区全序列,为猕猴进化关系研究及mtDNA控制区功能研究奠定基础。
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| 关 键 词： | 猕猴 线粒体DNA 控制区 全序列 |
Determination of the Complete Sequence of mtDNA Control Region in Macaca mulatta from Yunnan Province |
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| YU Wen-hai,HUANG Fen,YANG Feng-mei,LU Shuai-yao,ZHAO Yuan,SHEN Dong,WANG Jun-bin,CHEN Li-xiong,HE Zhan-long.Determination of the Complete Sequence of mtDNA Control Region in Macaca mulatta from Yunnan Province[J].Chinese Journal of Comparative Medicine,2011,21(12):42-45. |
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| Authors: | YU Wen-hai HUANG Fen YANG Feng-mei LU Shuai-yao ZHAO Yuan SHEN Dong WANG Jun-bin CHEN Li-xiong HE Zhan-long |
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| Institution: | YU Wen-hai1,HUANG Fen2,YANG Feng-mei1,LU Shuai-yao1,ZHAO Yuan1,SHEN Dong1,WANG Jun-bin1,CHEN Li-xiong1,HE Zhan-long1(1.Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Kunming 650118,China,2.Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650224,China) |
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| Abstract: | Objective To determine the complete sequences of the mitochondrial DNA control region in Macaca mulatta from Yunnan province.The complete sequences were analyzed for phylogenetic tree and nucleotide composition.Methods The complete sequences was amplified by polymerase chain reaction(PCR).The nucleotide composition was analyzed by comparison with the known control region sequence(AY612638) downloaded from GenBank database.The homology,the rate of transition/transversion and phylogenetic tree constructed by ... |
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| Keywords: | Macaca mulatta mtDNA Control region Complete sequence |
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