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| 维甲酸体外诱导大鼠胚胎垂体生长激素细胞的分化 | |
| 引用本文： | 张广会,吕文明,贾文志,王玉翠,杨光,蔺友志,李国忠,初明,傅松滨,程玉.维甲酸体外诱导大鼠胚胎垂体生长激素细胞的分化[J].中国微侵袭神经外科杂志,2011,16(12):560-562. |
| 作者姓名： | 张广会 吕文明 贾文志 王玉翠 杨光 蔺友志 李国忠 初明 傅松滨 程玉 |
| 作者单位： | 1. 150001,哈尔滨医科大学附属第一医院神经外三科 2. 150001,哈尔滨医科大学附属第一医院妇产科 3. 150081,哈尔滨医科大学遗传教研室 |
| 基金项目： | 黑龙江省自然科学基金，黑龙江省卫生厅基金，黑龙江省教育厅基金，哈医大一院基金，教育部高等学校博士学科点专项科研基金 |
| 摘 要： | 目的 探讨维甲酸(RA)在体外诱导大鼠胚胎垂体生长激素(GH)细胞的分化作用.方法 原代培养大鼠胚胎垂体细胞,采用不同浓度的维甲酸(10-8、10-7、10-6、10-5 mol/L)诱导大鼠胚胎垂体细胞分化,以DMEM培养基为作为对照组,诱导2、4、6d后,利用免疫组化检测GH细胞百分比,放射免疫分析检测细胞上清GH... |
| 关 键 词： | 垂体 生长激素 维甲酸 体外诱导 分化 鼠 |
Retinoic acid induces the differentiation of fetal rat pituitary somatotroph cells in vitro |
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| Zhang Guanghui,Lv Wenming,Jia Wenzhi,Wang Yucui,Yang Guang,Lin Youzhi,Li Guozhong,Chu Ming,Fu Songbin,Cheng Yu.Retinoic acid induces the differentiation of fetal rat pituitary somatotroph cells in vitro[J].Chinese Journal of Minimally Invasive Neurosurgery,2011,16(12):560-562. | |
| Authors: | Zhang Guanghui Lv Wenming Jia Wenzhi Wang Yucui Yang Guang Lin Youzhi Li Guozhong Chu Ming Fu Songbin Cheng Yu |
| Institution: | Zhang Guanghui1,Lv Wenming1,Jia Wenzhi1,Wang Yucui2,Yang Guang1,Lin Youzhi1,Li Guozhong1,Chu Ming1,Fu Songbin3,Cheng Yu1 1.Department of Neurosurgery,2.Department of Obstetric and Gynaecology,First Affiliated Hospital,Harbin Medical University,Herbin,Heilongjiang 150001,China,3.Department of Medical Genetics,Heilongjiang 150081 |
| Abstract: | Objective To explore effect of retinoic acid(RA) in inducing the differentiation of fetal rat pituitary somatotroph cells in vitro.Methods Primary fetal rat pituitary cells were cultured and treated with different concentrations of RA(10-8,10-7,10-6,10-5 mol/L) to induce the differentiation of growth hormone(GH) cells,and the cells treated with DMEM served as the control group.Immuno-histochemistry was used to detect the percentage of GH cells and radioimmunoassay to detect secretion of GH in supernatant 2,... |
| Keywords: | pituitary growth hormone tretinoin induction in vitro differentiation rats |
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