组织工程上皮移植对碱烧伤角膜新生血管的影响

目的：评价组织工程上皮移植在碱烧伤角膜缘干细胞缺乏症中对角膜新生血管的抑制作用。

方法：回顾性非随机的病例研究。2006/2011年我院收治的19例(23眼)完全性角膜缘干细胞缺乏症的碱烧伤患者， 10例13眼行组织工程上皮移植，9例10眼行羊膜移植。所有患者在手术前后均用裂隙灯观察角膜新生血管情况，在术后第21，60d对角膜新生血管进行评分比较。

结果：术后第21d和术后第60d组织工程上皮移植组和羊膜移植组角膜新生血管均较术前明显减少( P<0.05)，在术后两个评价时间点，组织工程上皮移植组平均角膜新生血管分数明显低于羊膜移植组。

结论：对碱烧伤所致角膜缘干细胞缺乏的患者，组织工程上皮移植抑制角膜新生血管的作用明显好于羊膜移植。

Tissue-engineered epithelium transplantation on corneal neovascularization in alkali-induced limbal stem cell deficiency

AIM: To evaluate the clinical outcomes of tissue-engineered epithelium transplantation on decreasing corneal neovascularization in patients with limbal stem cell deficiencies caused by alkali burns.

METHODS: A non-randomized, retrospective observational case series included 23 eyes of 19 cases with severe corneal alkali burns from 2006 to 2011 in our hospital. All of these eyes had complete limbal stem cell deficiencies. Ten cases(13 eyes)were performed with tissue-engineered epithelium transplantation, and 9 cases(10 eyes)were performed with amnion membrane transplantation. Vascularization was observed in all cases by slit-lamp microscope before and after surgery. A neovascularization scoring system was used to evaluate each eye at the 21^th and 60^th after surgery.

RESULTS: At the 21^th day and 60^th day after tissue-engineered epithelium transplantation or amniotic membrane transplantation was performed, corneal neovascularization decreased significantly(P<0.05). And averaged scores of corneal neovascularization were significantly lower in tissue-engineered epithelium transplantation group at the 21^th day and 60^th day after surgery than those of amniotic membrane transplantation group(P<0.05).

CONCLUSION: Tissue-engineered epithelium transplantation is more effective in inhibitting the growth of corneal neovascularization in patients with limbal stem cell deficiencies caused by alkali burn than amnion membrane transplantation.