中药白疕合剂对实验性银屑病小鼠模型的影响

目的观察中药白疕合剂对银屑病模型小鼠阴道上皮细胞有丝分裂及白细胞介素8(interleukin-8,IL-8)产生的影响,并探讨其作用机制。方法将50只实验性银屑病模型小鼠随机分为5组,连续用药灌胃4周后,采用小鼠阴道组织HE染色法观察白疕合剂对上皮细胞有丝分裂指数的影响,采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)法检测各组小鼠血清中IL-8的水平,并与10只正常小鼠作对比。结果白疕合剂能显著抑制银屑病小鼠模型阴道上皮有丝分裂,降低血清中IL-8的产生(与对照组比较,P0.01)。结论白疕合剂可能通过抑制上皮细胞有丝分裂,降低血清IL-8的产生发挥治疗银屑病的作用。

Effect of White Mange Medicine Mixture on Mice Models of Psoriasis

Objective To investigate the mechanism of White Mange medicine mixture on the mice models of psoriasis,and to observe its effects on mitosis index and serum chemokine IL-8.Methods 50 mice models of psoriasis were divided into five groups randomly.After four weeks medication gavage consecutively,the effect of White Mange mixture on mitosis exponential of vaginal epithelial cells was measured by HE staining,the production of IL-8 was detected by enzyme-linked immuno sorbent assay(ELISA),and 10 healthy mice were tested as controls.Results White Mange mixture could significantly inhibiting the vaginal epithelial mitosis of mice models of psoriasis,decreasing the expression of serum IL-8.(compared with control group,P<0.01).Conclusion White Mange mixture plays a role in the treatment of psoriasis,and it may via the inhibition of vaginal epithelial mitosis and the decreasing of serum IL-8.