| 活性氧致正常精子膜脂质过氧化作用分析 |
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| 引用本文： | 商学军,熊承良,黄宇烽.活性氧致正常精子膜脂质过氧化作用分析[J].华中科技大学学报(医学版),2002,31(6):612-614. |
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| 作者姓名： | 商学军 熊承良 黄宇烽 |
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| 作者单位： | 1. 华中科技大学同济医学院计划生育研究所,武汉,430030
2. 南京军区南京总医院生殖遗传研究室,南京,210002 |
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| 基金项目： | 湖北省计生委科研基金资助项目 (HJ2 0 0 10 3) |
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| 摘 要： | 为探讨活性氧 (ROS)致正常精子膜损伤机理 ,采用硫巴比妥酸法 (TBA)检测 ROS攻击后精子膜脂质过氧化产物— MDA。结果发现 :与对照组相比 ,ROS攻击后的正常精子膜脂质过氧化损伤明显加重 ,MDA含量显著增高(P<0 .0 0 1)。提示 :ROS可导致精子膜脂质过氧化损伤 ,影响正常精子功能
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| 关 键 词： | 活性氧 精子 脂质过氧化 ROS 硫巴比妥酸法 |
| 修稿时间： | 2001年9月18日 |
Reactive Oxygen Species-induced Lipid Peroxidation in Normal Sperm Membrane |
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| Shang Xuejun ,Xiong Chengliang ,Huang Yufeng Institute of Family Planning Research,Tongji Medical College,Huazhong University of Science and Technology,Wuhan Laboratory of Reproduction & Genetics,Nanjing General Hospital of Nanjing Command,PLA,Nanjing.Reactive Oxygen Species-induced Lipid Peroxidation in Normal Sperm Membrane[J].Journal of Huazhong University of Science and Technology(Health Sciences),2002,31(6):612-614. |
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| Authors: | Shang Xuejun Xiong Chengliang Huang Yufeng Institute of Family Planning Research Tongji Medical College Huazhong University of Science and Technology Wuhan Laboratory of Reproduction & Genetics Nanjing General Hospital of Nanjing Command PLA Nanjing |
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| Institution: | Shang Xuejun 1,Xiong Chengliang 1,Huang Yufeng 2 1 Institute of Family Planning Research,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030 2 Laboratory of Reproduction & Genetics,Nanjing General Hospital of Nanjing Command,PLA,Nanjing 210002 |
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| Abstract: | To study the mechanism of normal sperm damage induced by reactive oxygen species (ROS), malondialdehyde (MDA) was measured by thiobarbituric acid method after spermatozoa was attacked by ROS. Compared with control group, after spermatozoa in the treatment group were incubated with ROS, damage of lipid peroxidation in sperm membrane was significantly aggravated and concentration of MDA was significantly increased ( P <0 001). It suggested that ROS could induce damage of lipid peroxidation in sperm membrane and influence normal sperm functions. |
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| Keywords: | reactive oxygen species spermatozoa lipid peroxidation |
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