咖啡因与苯巴比妥联合对顺铂的体外抗癌增效作用

目的：研究咖啡因（CAF）对顺铂（DDP）的抗癌增效作用及苯巴比妥（PBT）是否抑制咖啡因的抗癌增效作用。方法：以SPC－A－1细胞为,要用MTT法研究甲基黄嘌呤类药物－CAF及其与PBT联合应用对DDP的体外细胞毒作用的影响,结果：120ug/ml的CAF及10ug/ml PBT对细胞生长无明显的细胞毒作用,120ug/ml的CAF可明显增强DDP对SPC－A－1细胞的细胞毒作用（P＜0．05）,10ug/ml PBT对DDP无增效作用（P＞0．05）,120ug/ml CAF与10ug/ml PBT的混合物也能增强DDP的细胞毒作用（P＜0．01）,其增效作用与单纯CAF的增效作用比较无显著性差异（P＞0．05）,。结论：咖啡因可明显增强DDP对SPC－A－1细胞的体外抗癌效应,而苯巴比妥对咖啡因的体外抗癌增效作用无拮抗作用,提示苯巴比妥作为降低咖啡因副反应的成分,有可能与咖啡因联合作用作为化疗增效剂用于肺癌治疗。

Enhancement of the anticancer activity of cisplatin combined with caffeine and phenobarbital in vitro

Objective　To investigate the enhancing effect of caffeine(CAF) on the anticancer activity of cisplatin on human lung cancer SPC-A-1 cells in vitro and study whether this enhancing effect of caffeine could be inhibited by phenobarbital(PBT). Methods　The enhancing effect of combination of caffeine and phenobarbital on the cytotoxicity of DDP on SPC-A-1 was detected by MTT assay. Results　No significant cytotoxicity was observed in SPC-A-1 cells treated with 120*!μg／ml CAF alone, 10*!μg／ml PBT alone or both of them. CAF with a concentration of 120*!μg／ml showed significant enhancing effect on the cytotoxicity of DDP on SPC-A-1 (P＜0.05), but PBT of 10*!μg／ml did not (P＞0.05). The combination with CAF of 120*!μg／ml and PBT of 10*!μg／ml could remarkably enhance the cytotoxicity of DDP too (P＜0.01). The enhancing effect of CAF plus PBT on cytotoxicity of DDP was not significantly different from that of CAF (P＞0.05). Conclusion　CAF can enhance anticancer activity of DDP in SPC-A-1, which is not antagonized by PBT. The results suggest that as a composition to reduce the side effects of CAF in vitro, PBT may be used as an enhancing agent of chemotherapeutical drugs together with CAF in the treatment of lung cancer.