子宫颈鳞癌组织中基质金属蛋白酶2的表达及其意义

目的:探讨基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)与宫颈鳞癌的发生、发展及转移的关系.方法:用免疫组织化学链霉素抗生物素蛋白-过氧化物酶法(SP法)测定MMP-2的表达及分布;用明胶酶谱法测定活性型MMP-2蛋白的含量;用RT-PCR技术检测MMP-2 mRNA表达水平.结果:在宫颈鳞癌组织(squamouscell carcinomas,SCC)中MMP-2的阳性表达率为81.13%(43/53),高于在重度鳞状上皮细胞内损害(high-gradesquamous intraepithelial lesions,HSIL,47.62%,10/21)及轻度鳞状上皮细胞内损害(low-grade squamous intraepithe-lial lesions,LSIL,10.00%,1/10))中的表达;而HSIL则高于LSIL(P<0.05).淋巴结转移组MMP-2的阳性率(91.30%)高于非淋巴结转移组(73.33%,P<0.05),但其表达在不同的病理分级与临床分期之间差异无统计学意义.在官颈鳞癌组织中MMP-2蛋白酶活性[(5.81±2.17)×10⁴]高于HSIL[(2.28±0.83)×10⁴]和LSIL[(1.94±0.71)×10⁴,P<0.01,P<0.01].MMP-2 mRNA在宫颈鳞癌中的表达量(0.87±0.44)亦高于HSIL(0.46±0.22)和ISIL(0.37±0.20)中的表达量.结论:MMP-2与宫颈鳞癌的发生、发展及转移有关,MMP-2表达量的增高,尤其是其酶活性的增高可作为判断宫颈鳞癌具有浸润、转移倾向的临床参考指标.

Expression of MMP-2 and its clinical significance in cervical squamous carcinoma

Objective:To investigate the relationship of the expression of matrix metalloproteinase-2(MMP-2) with tumorigenesis,development and metastasis of cervical squamous carcinoma.Methods:The expression and distribution of MMP-2 protein were detected by using immunohistochemical SP method.The active protein and mRNA of MMP-2 were determined by using gelatin Zymography and RT-PCR,respectively.The relationships between those indexes and the factors related to clinical pathology of cervical carcinoma were analyzed s...