Analysis of Yaba tumor poxvirus-induced proteins in infected cells by two-dimensional gel electrophoresis

Lack of inhibition of host protein synthesis by Yaba tumor poxvirus and comigration of viral and host proteins did not interfere with high resolution of virus-induced proteins when two-dimensional gel electrophoresis was employed. A total of 84 virus-induced proteins, 44 structural and 40 nonstructural, comprising 62.9% of the coding capacity of Yaba virus DNA, were detected. These proteins may be further categorized in two classes: early, synthesized in the presence of DNA inhibitor, Ara-c; and late, beginning at 6 hr p.i. with the bulk of viral protein synthesis occurring later than 30 hr p.i. The rate of synthesis of viral proteins fell into two patterns: group A, in which the rate of synthesis decreased; and, group B, in which the rate of synthesis increased after the initial appearance of the protein.