喉癌发生中人乳头瘤病毒感染与Langerhans细胞、p53表达的关系

目的：探讨喉癌的发生机理。方法：应用免疫组化ＬＳＡＢ法对３０例喉鳞状细胞癌人乳头瘤病毒（ＨＰＶ）感染、Ｌａｎｇｅｒｈａｎｓ细胞（ＬＣ）及ｐ５３蛋白表达进行了研究。结果：２６．７％的病例可以检测到ＨＰＶ抗原成分。ＨＰＶ感染的癌旁粘膜内ＬＣ数量明显少于无感染者，且形态也发生改变。ｐ５３蛋白表达阳性率在ＨＰＶ感染组（３７．５％）明显低于ＨＰＶ检测阴性组（８３．３３％）。结论：提示ＨＰＶ、ＬＣ、ｐ５３在喉癌发生发展过程中起一定作用，且相互影响，ＨＰＶ感染引起ＬＣ数量减少，局部免疫功能降低，ＨＰＶ感染还可能通过表达的肿瘤蛋白或其他机制使抑癌基因ｐ５３失活，进而导致肿瘤的发生。

The relation between human papilloma virus infection and Langerhans cell, expression of p53 in carcinogenesis of laryngeal carcinoma

Purpose To investigate the carcinogenesis of laryngeal carcinoma. Methods HPV infection, Langerhans cell (LC) and p53 protein expression were evaluated in 30 cases of squamous cell carcinoma of laryhx by LSAB immunohistochemical technique. Results HPV antigen positivity rate was 26 7%. The amount of LCs in pericarcinoma mucosa with HPV infection was much less than that without HPV infections, and LC changed morphologically. Positivity rate of p53 expression in HPV infected group (37 5%) was significantly lower than that in no HPV infected group (83 33%). Conclusions HPV, LC and p53 gene play a role in carcinogenesis and development of laryngeal carcinoma, and interact on each other. HPV infection may result in LC diminishment and deficiency of the local immune function. HPV can make p53 gene inactivation through expression certain kind of tumor protein so that it come out carcinogenesis.