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目的 探讨干扰素调节因子（IRF）－1在实验性自发免疫性甲状腺炎（EAT）发病及进展过程中的作用。方法 对6周龄NOD小鼠用佐剂混合的小鼠甲状腺球蛋白（mTg)免疫，4周后用相同剂量mTg加强免疫。再过4周取甲状腺和血清样本。甲状腺组织做HE染色，分析EAT的发病率及重症度。应用酶免疫测定（EIA）法测定血清T4值，评估甲状腺功能。采用酶联免疫吸附测定（ELISA）法测定血清抗小鼠甲状腺球蛋白抗体（anti-mTgAb)值，分析其结果，探讨与EAT重症度的关系。结果 Tg免疫组IRF－1＋／＋，＋／－，－／－小鼠均比对照组显示EAT的高发倾向。在EAT重症度上，Tg免疫组比对照组明显增加，差异有显著的统计学意义，但与IRF－1基因型无相关性。Tg免疫组anti-mTgAb值比对照组上升，有统计学意义，但在IRF－1基因型之间未显示相关性。血清anti-mTgAb值与EAT重症度之间显示正相关关系。Tg免疫组血清T4值比对照组降低，有统计学意义，但在IRF－1基因型之间未显示相关性。结论 IRF－1－／－小鼠与IRF－1＋／＋，＋／－小鼠均可诱发Tg免疫性EAT，说明在Tg免疫性EAT的发病过程中IRF－1的作用不大，证明IRF－1缺陷NOD小鼠在桥本病模型的病态分析中非常有价值。

The role of interferon regulatory factor in the etiology of autoimmune thyroiditis

Objective To investigate the role of interferon regulatory factor(IRF)-1 in the development of experimental autoimmune thyroiditis(EAT). Methods Either thyroglobulin(Tg) or saline emulsified in an adjuvand was administered to mice at 6 and 10 weeks after birth. Thyroid tissue and sera were obtained from 14-week-old mice. HE staining has been emplyed to evaluate the incidence and severity of EAT.Serum anti-mouse thyroglobulin antibodies(anti-mTgAb) titers of mice was determined by ELISA and the relationship between EAT severity and anti-mTgAb titer was analyzed. The value of T4 in serum was determined by EIA for evaluation of the thyroid function.Results Tg-treated IRF-1+/+,+/-and-/-mice revealed higher incidence of the EAT than that of the control group.EAT severity of Tg-treated mice was significantly higher than that of the controlled, but was independent of the genotype of IRF-1. Anti-mTgAb titer of Tg-treated mice was significantly higher than that of the controlled, but was independent of the genotype of IRF-1. Serum anti-mTgAb titers was in proportion to the severity of the EAT. Serum T4 value of Tg-treated mice was significantly lower than that of the controlled,but was independent of the genotype of IRF-1.Conclusion Tg-immunised EAT could be induced in IRF-1-/-and+/+,+/-mice. These results suggest that IRF-1 plays a minor role in the pathogenesis of immunological EAT and IRF-1 lacking NOD mouse plays an important role in the analyses of the model of the Hashimoto's morbidity.