反相高效液相色谱法同时检测人血浆利多卡因和罗哌卡因

目的：建立人血浆利多卡因和罗哌卡因的高效液相色谱分析方法。方法：高效液相色谱仪为Agilent 1100系列;血浆碱化后经乙酸乙酯萃取浓缩,以ZORBAX Eclipse XDB-C18（4.6 mm×150 mm,5μm,Agilent,USA）为色谱柱;以乙腈∶0.02 mol·L^-1 KH2PO4（16∶84,V/V）为流动相,流速为1.0 ml·min^-1;检测波长为210 nm;内标为布比卡因。结果：利多卡因和罗哌卡因浓度在0.05～10 mg·L^-1范围内线性关系良好（r=0.999）;最低检测限为0.05 mg·L^-1;两种药物高、中、低3个浓度的平均相对回收率分别为（99.79±2.94）%和（100.32±2.95）%;平均绝对回收率分别为（68.36±4.63）%和（70.87±4.88）%;日内精密度变异系数分别为（0.86±0.43）%和（1.36±0.77）%,日间精密度变异系数分别为（2.07±1.45）%和（1.43±0.23）%.结论：本方法快速、简便、灵敏、准确可靠,适用于血浆利多卡因和罗哌卡因的同时测定及其临床药代动力学研究。

Simultaneous determination of Lidocaine and Ropivacaine by antiphase high performance liquid chromatography in human plasma

Objective： To develop a high performance liquid chromatography method for the simulta neous determination of Lidocaine and Ropivacaine in human plasma. Methods： High performance liquid chromatography was equipped with Agilent 1100 series. Lidocaine and Ropivacaine were extracted from plasma with acetic ether after alkalization. The analytical colunm was packed with ZORBAX EclipseXDB-C18（4.6 mm × 150 mm, 5 μm, Agilent, USA）. The mobile phase was consisted of acetonitrile-potassium phosphate buffer （0.02） （16：84, V/V） and the flow rate was 1ml·min^-1. The UV detection wavelength was 210nm. The internal standard was Bupivacaine. Results： Excellent linear relationship was obtained from the range of 0.05 mg ·L^-1 to 10 mg·L^-1（r=0.999）. The limit determination of Lidoaaine and Ropivacaine was 0.05 mg·L^-1 and the average relative recovery were （99.79 ± 2.94）% and （100.32 ± 2.95）%, respectively. The average absolute recovery were （68.36 ± 4.63）% and （70.87 ± 4.88）%, respectively. The intra-day precision RSD were （0.86±0.42）% and（1.36±0.77）%, respectively ; the inter-day precision RSD was （2.07± 1.45）% and （1.43± 0.23）%, respectively. Conclusion：The method is simple, rapid, and accurate. It can be used to determine the Lidocaineand Ropivacaine concentration simultaneously in human plasma and for studying of their pharmacokinetics.