人体静脉血样采集管的不同内表面状态对血小板活化的影响

目的 研究人体静脉血样采集管的不同内表面状态对血小板活化的影响.方法 用聚环氧烷-聚二甲基硅氧烷共聚物(L722)、硅烷偶联剂对塑料管(PET)和玻璃管制膜,对L722玻璃管、L722 PET管、玻璃管、PET管、硅烷偶联剂制膜玻璃管及聚丙烯管(PP)内表面进行接触角分析.用上述材料的血样采集管采集血液标本并在室温下滚动混匀孵育110～60 min.用流式细胞术(FCM)检测血小板激活标志物CD62p.结果 不同材质及表面处理血液采集管的内表面的接触角大小在一定程度上反映了血小板活化率,但并不呈线性关系.PET管经L722表面改性后表达CD62p阳性的活化血小板百分率由(37.4±14.8)%下降到(21.9±12.4)%.玻璃管对表达CD62p阳性的活化血小板百分率为(54.5±18.6)%,明显大于PET管.玻璃管制膜后对血小板的激活明显减少,用硅烷偶联剂制膜的玻璃管表达CD62p阳性的活化血小板百分率为(28.3±8.2)%,明显低于L722制膜玻璃管.用PET作为基体材料经过L722表面处理后对血小板活化明显低于L722制膜的玻璃管的(41.5 ±15.9)%和用硅烷偶联剂制膜的玻璃管的(22.0±12.8)%.不同管对血小板活化时间进程显示:60 min L722 PET管和聚丙烯管的血小板活化与30 min的结果差异无统计学意义.结论 不同材质及表面处理血液盛装管所导致的不同表面能状态对血小板活化的影响有明显差异,硅油表面处理能有效改善血液采集管的血液相容性.FCM检测CD62p是评价血液收集管材料介导的血小板活化的灵敏指标,对建立血液盛装材料表面处理模型及筛选临床应用材料具有重要意义.

Effects of different inner face of human venous blood container on platelet activation

Objective To study the effects of different inner face status of human venous blood container on platelet activation. Methods The plastic( polyethene terephthalate,PET) and glass tubes were coated with polyalkyleneoxide modified polydimethylsiloxane(L722). The contact angles of L722-coated glass and L722- coated PET tubes, glass tubes, PET tubes, silane coupling agent-coated glass tubes and polypropylene (PP) tubes were analyzed respectively. The blood were drawn into above tubes, and then incubated in a roller bottle at room temperature for 10-60 mira The marker of activated platelete, CD62p, was detected by flow cytometry(FCM). Results The inner face contact angle of the blood collection tubes with different material and surface treatment reflected platelet activation to a certain extent, but was not linear. The percent age of CD62p positive platelets in L722-modified PET tubes reduced from (37.4 ±14. 8) % to(21.9 ± 12. 4) %. The platelet activation by glass tubes was (54.5 ± 18.6 ) %, markedly more than PET tubes. While membrane formed, the platelet activation by glass tubes decreased remarkably, and the percentage of CD62p positive platelet in silane coupling agent-coated glass tubes were ( 28. 3 ± 8.2 ) %,markedly less than that of the L722-coated glass tubes. The platelet activation by PET-based material ectally modified with L722 was obviously less than L722-coated glass tubes and the percentage of CD62p positive platelet in silane coupling agent-coated glass tubes were ( 41.5 ± 15.9 ) % and ( 22. 0 ± 12. 8 ) %,respectively. The time course of platelet activation by different tubes showed that the platelet activation by L722-coated PET tubes and polypropylene tubes in 60 rain was not significantly different from the results in30 mitt Conclusions The diverse surface energy status induced by different material and surface treatment of blood collection tubes have obvious effects on the activation of platelets. The silicic oil surface treatment can effectively improve the blood compatibility of blood collection tubes. CD62p detectod by FCM is a sensitive marker for the evaluation of the activation of platelets induced by the material of blood collection tubes. It is of importance in the establishment of surface treatment model of blood container and screening out the material of clinical application.