康莱特对系膜细胞增殖及端粒酶活性影响的研究

目的:探讨康莱特注射液(KLT)对大鼠系膜细胞(MC)增殖及端粒酶活性的影响。方法:采用活细胞计数法检测不同浓度KLT对MC增殖的影响,采用MTT法检测MC在KLT,IL1(白细胞介素1)分别及共同刺激下的增殖情况,采用端粒重复序列扩增酶联免疫吸附法及聚丙烯酰胺凝胶电泳银染法进行端粒酶活性定量及定性检测并作相关统计学检验。结果:KLT抑制MC增殖,伴随端粒酶活性降低。IL1刺激MC增殖,伴随端粒酶活性升高;两者先后共同作用,抑制MC增殖,抑制端粒酶活性。结论:康莱特对系膜细胞的增殖和端粒酶表达均有抑制作用,并可干预IL1的刺激效应,对防治MC增殖相关的肾小球肾炎可能有应用价值。

The effect of Kanglaite injection(KLT) on the proliferation and telomerase activity of rat mesangial cells

Objective: To observe the effect of Kanglaite injection(KLT) on the proliferation and telomerase activity of mesangial cells in rats. Method: MTT, telomere repeat amplification protocal(TRAP), ELISA, PAGE and silver-stain were applied to detect the growth rate and telomerase activity of MC after stimulation of KLT and IL-1. Result: The growth rate of MC was enhanced by IL-1 stimulation, which was accompanied with a redection of the activity of telomerase. Adversely, the growth rate of MC was reduced by KLT, which was accompanied with an enhancement of activity of telomerase. Moreover, the grouth rate of MC and the activity of telomerase were both inhibited by the combinative use of IL-1 and KLT without any influence from the sequence of their administration. Conclusion: KLT could inhibit proliferation and telomerase activity of MC with or without pre-stimulation with IL-1. KLT might be useful to prevent and treat glomerular nephritis related to MC proliferation.