结核分枝杆菌ClpC2基因序列分析及功能预测

目的对结核分枝杆菌的基因clpC2（Rv2667）的同源基因进行序列分析和相似性比较，并对其编码蛋白进行理化性质及功能的预测，为CIpC2蛋白的结构与功能研究提供基础技术资料。方法根据Gene Bank中分枝杆菌clpC2同源基因的核苷酸序列，进行多重序列比对和进化树构建；应用ExPASy在线序列分析工具，对结核分枝杆菌CIpC2蛋白质理化性质及保守结构域进行预测；应用Geneontology（GO）在线软件对ClpC2蛋白酶的功能进行分析。结果clpC2系统进化树分析，与16SrRNA基因相比，进化距离明显变大，而在结核分枝杆菌复合群中高度保守。ClpC2蛋白为亲水性非跨膜蛋白，在细胞质表达，具有水解和催化功能，是一个依赖于ATP的Clp亚单位。结论clpC2基因在结核分枝杆菌复合群中高度保守，其编码的蛋白ClpC2是一个依赖于ATP的水解蛋白酶。

Sequence analysis of Mycobacterium tuberculosis ClpC2 and its function predic- tion

Objective To provide evidence for further research on the structures and functions of Rv2667 gene encoding ClpC2 protein in Mycobacterium tuberculosis, this study analyzed and predicted the physical and chemi- cal characteristics of Mycobacterium tuberculosis ClpC2, and compared its nucleotide sequence similarity among myeobacteria. Methods According to the NCBI clpC2 gene sequence of Mycobacterium tuberculosis, multiple se- quence alignment and phylogenetic - tree - construction were performed. Physicochemical properties and con- served domain of ClpC2 protein in Mycobacterium tuberculosis was predicted with ExPASy online tools; The func- tion of ClpC2 protease was analyzed by Gene Ontology （GO） online software. Results Compared with the 16s rRNA gene, evolutionary distance is significantly bigger. ClpC2 protein is hydrophilic, a non -transmembrane protein, expressed in endoplasmic reticulum, with hydrolysis and catalytic function, and an ATP -independent Clp subunit. Conclusion ClpC2 is conservative highly in Mycobacterium tuberculosis complex, and its encoding protein is an ATP- independent proteolytic enzyme.