人骨髓源性成骨细胞体外培养和生物学特性的研究

目的研究人骨髓源性成骨细胞的生物学特性，为骨组织工程选择理想的种子细胞提供依据。方法采用全骨髓培养法培养骨髓基质干细胞，传代扩增后采用诱导培养基进行诱导，对骨髓基质干细胞诱导成的成骨细胞进行细胞形态学观察，检测碱性磷酸酶活性及分泌钙结节和Ⅰ型胶原的能力。结果骨髓基质干细胞可以进行传代和大量扩增，经诱导培养后能够诱导出成骨细胞，诱导出的成骨细胞具有典型成骨细胞的形态学和生物学特性。结论骨髓源性成骨细胞具有良好的成骨细胞特性，可以作为骨组织工程的种子细胞。

The culture in vitro and biological characteristics study of osteoblasts derived from human bone morrow

Objective To study biological characteristics of osteoblasts derived from human bone morrow and to provide proof for bone tissue engineering selecting ideal seed cells. Methods The bone marrow mesenchymal stem cells ( MSCs) were cultivated with complete bone marrow,and were propagated and induced in the inducement culture medium. The pheno-type of osteoblasts derived from bone marrow was examined both morphologically and biologically. Results The osteoblasts could be induced from human bone morrow mesenchymal stem cells,which had typically morphological and biological characteristics of osteoblasts. Conclusion The osteoblasts derived from human bone marrow have the entire characteristics of osteo-blast, thus they can be used as seed cells of bone tissue engineering.