蔬菜中农药残留的样品前处理方法的研究

目的]建立一种简便易行,适用于检测蔬菜中4大类农药残留的气相色谱分析样品前处理方法。方法]以甲胺磷、对硫磷作为有机磷农药代表,灭多威为氨基甲酸酯类农药代表,氯菊酯为菊酯类农药代表,采用液-液萃取、索氏提取、国标固相萃取法、本法的固相萃取法4种前处理方法处理样品,按GB/T 5009.145-2003和GB/T 5009.146-2003法测定并对不同的前处理方法的测定结果进行比较。结果]该方法所检测的农药的最低检出限为0.002～0.05 0;加标回收率为86.3%～104.9%;相对标准偏差为3.92%～11.08%;校正曲线相关系数为0.997 9～0.999 8。结论]与国标前处理方法比较,它在保证国标方法要求的精密度和准确性的同时,具有检验时间减少、前处理试剂消耗少、空白的背景干扰小等特点。

Research on the Sample Pretreatment Methods of Pesticide Residues in Vegetables

Objective]To establish a sample pretreatment method,which is correct,sensitive,and suitable for the detection of four types of pesticide residues in vegetables by gas chromatography.Methods]Samples were processed by liquid-liquid extraction,soxhlet extraction,solid phase extraction method and the method.Comparison was made on the measuring results for the determination of the different pretreatment methods.Results]The lowest detection limit of the method of the detection of pesticides was 0.002-0.050.The standard recovery was 86.3%-104.9%.The relative standard deviation was 3.92%-11.08%.The correction curve correlation coefficient was 0.997 9-0.999 8.Conclusion]The processing method compared with the GB code method,while guaranteeing the GB code method’s precision and accuracy,has the proving time reduced,the reagent consuming reduced in the pretreatment,few disturbs in the blank background,and other characteristics.