LncRNA-MAFG-AS1在结直肠癌中的表达及临床意义

目的 探讨LncRNA-MAFG-AS1在结直肠癌中的表达及临床意义。方法 选取我院行手术切除的结直肠癌组织及对应的癌旁正常组织各80例，应用定量即时聚合酶链锁反应(quantitative real time polymerase chain reaction, qRT-PCR)检测结直肠癌组织及癌旁正常组织中LncRNA-MAFG-AS1的表达水平，并分析其与结直肠癌临床病理特征之间的关系，所有患者随访截止日期到2019年12月31日，应用Kaplan-Meier(K-M)法进行生存分析，采用Cox回归模型分析结直肠癌预后的危险因素。结果 结直肠癌组织中LncRNA-MAFG-AS1相对表达量为2.398±0.214,高于癌旁正常组织的1.032±0.132,差异有统计学意义(P<0.05)。当患者TNM分期更高、中高分化及存在淋巴结转移和远期转移时，LncRNA-MAFG-AS1过表达(P<0.05)。K-M生存分析显示高表达患者无进展生存时间(progression-free survival, PFS)为(20.36±3.21)个月，低表达患者为(29.71±4....

Expression and clinical significance of LncRNA-MAFG-AS1 in colorectal cancer

Objective To investigate the expression and clinical significance of LncRNA-MAFG-AS1 in colorectal cancer (CRC).Methods A total of 80 cases of CRC tissues that were surgically removed in our hospital and corresponding adjacent normal tissues were selected. The expression levels of LncRNA-MAFG-AS1 in CRC tissues and adjacent normal tissues were detected by qRT-PCR, and their relationship with the clinicopathological features of CRC was analyzed. All patients were followed up until December 31, 2019. Kaplan-Meier (K-M) method was used for survival analysis, and Cox regression model was used for risk factor analysis of the prognosis of CRC.Results The relative expression of LncRNA-MAFG-AS1 in CRC tissues was 2.398±0.214, which was higher than that in adjacent normal tissues 1.032±0.132, with a statistical difference (P＜0.05). The overexpression of LncRNA-MAFG-AS1 was associated with higher TNM stage, moderate and high differentiation, and the presence of lymph node metastasis and long-term metastasis (P＜0.05). K-M survival analysis showed that the progression-free survival (PFS) was (20.36±3.21) months in patients with high expression, and (29.71±4.23) months in patients with low expression; the overall survival (OS) was (38.69±6.24) months in patients with high expression, and (54.26±5.14) months in patients with low and high expression; the difference in PFS and OS between two groups was statistically significant (P＜0.05). Cox multivariate regression analysis showed that higher TNM stage, moderate and high differentiation, presence of lymph node metastasis, long-term metastasis and high expression of LncRNA-MAFG-AS1 were independent risk factors for the prognosis of CRC.Conclusion LncRNA-MAFG-AS1 is highly expressed in CRC tissue, and is related to the degree of differentiation, TNM stage, lymph node metastasis and long-term metastasis of CRC patients, which are independent risk factors affecting the prognosis of CRC patients.