小儿过敏性紫癜性肾炎SOCS-3及HMGB1与Th17/Treg细胞失衡的关系

目的 对儿童过敏性紫癜性肾炎(henoch-schonlein purpura nephritis, HSPN)细胞因子信号转导抑制蛋白3(suppressor of cytokine signaling-3,SOCS-3)及高迁移率族蛋白B1(high mobility group protein B1,HMGB1)与辅助性T细胞/调节性T细胞(helper T cell 17/regulate T cell, Th17/Treg)细胞失衡的关系进行探讨。方法 选取HSPN患儿100例作为研究对象，将其纳入HSPN组，同时选取同期在我院过敏性紫癜患儿100例作为对照组，分别于2组入院时抽取空腹静脉血，比较2组HMGB1、SOCS3 mRNA、Th17、Treg检测结果，并计算Th17/Treg水平，利用ROC分析HMGB1、SOCS3 mRNA、Th17、Treg、Th17/Treg预测患儿发生HSPN的价值，通过person系数分析HMGB1、SOCS3 mRNA与Th17、Treg、Th17/Treg的相关性。结果 HSPN组HMGB1、SOCS3 mRNA、Th17、Th17/...

The relationship between SOCS-3, HMGB1 and Th17/Treg cell imbalance in children with henoch-schonlein purpura nephritis

ObjectiveTo study the relationship between suppressor of cytokine signaling-3(SOCS-3), high mobility group protein B1(HMGB1) and Th17/Treg cell imbalance in children with Henoch-Schonlein purpura nephritis(HSPN).MethodsA total of 100 children with HSPN were selected as research subjects, and included into HSPN group. Meanwhile, 100 children with Henoch Schonlein purpura in our hospital during the same period were selected as the control group, On admission, fasting venous blood was taken from the two groups. The results of HMGB1, SOCS3 mRNA, Th17 and Treg were compared between two groups, and Th17/Treg levels were calculated. Receiver operating characteristic(ROC) curve was used to analyze the value of HMGB1, SOCS3 mRNA, Th17, Treg and Th17/Treg in predicting HSPN, and Person coefficient was used to analyze the correlation of HMGB1 and SOCS3 mRNA with Th17, Treg and Th17, Treg.ResultsIn HSPN group, HMGB1, SOCS3 mRNA, Th17, and Th17/Treg were significantly higher than those in control group, while Treg level was significantly lower than that in control group, with statistical difference(P＜0.05). ROC analysis showed that the area under the ROC curve(AUC) of HMGB1, SOCS3 mRNA, Th17, Treg, and Th17/Treg in predicting HSPN in children was 0.944, 0.924, 0.942, 0.973 and 0.955, respectively. According to correlation analysis, HMGB1 and SOCS3 mRNA were significantly and positively correlated with Th17 and Th17/Treg, and significantly and negatively correlated with Treg(P＜0.05).ConclusionThe expression of SOCS-3 and HMGB1 in children with HSPN is highly correlated with Th17/Treg cell imbalance.