Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of beta-hemolytic streptococci |
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| Authors: | Chunmei Zhou Lili Tao Bijie Hu Jian Ma Xiangru Ye Shenglei Huang Yan Ma Yuzhang Shan |
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| Affiliation: | 1.Department of Clinical microbiology laboratory, Zhongshan Hospital, Fudan University, Shanghai 200032, China; 2.Department of Respiratory Medicine, Huangdong Hospital, Fudan University, Shanghai 200040, China; 3.Department of Respiratory Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China; 4.Department of Respiratory Medicine, Yingzhou Hospital, Ningbo University, Ningbo 315040, China |
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| Abstract: | ObjectiveMatrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as promising technology for species identification. The purpose of this investigation was to compare the performance of MS and the traditional method for identification of beta-hemolytic streptococci (BHS).MethodsClinical BHS isolates were identified by the BD Phoenix SMIC/ID Streptococcal panels, and two MALDI-TOF MS platforms: the VITEK MS and the Bruker MALDI Biotyper systems respectively. In case of discordant results, 16sRNA sequencing was performed to provide the reference ID.ResultsA total of 96 isolates of BHS were analyzed. Thirty-six isolates (20.8%) were re-tested by BD Phoenix for identification failure; and four isolates (4.2%) were rerun on the Bruker system for low identification score. No isolate need a second run for identification by Vitek MS system. Overall, BD Phoenix, BioTyper and Vitek MS automated system accurately identified 76 strains (79.2%), 91 (94.7%) strains and 92 (95.8%) strains, respectively.ConclusionsOur study suggests that MALDI-TOF MS is a superior method to conventional phenotypic methods for BHS identification. |
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| Keywords: | Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) MS beta-hemolytic streptococci (BHS) identification |
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