白细胞介素10在银屑病中的表达特征及影响

目的：分析银屑病患者及银屑病小鼠模型皮损处白细胞介素10（IL-10）特点及其与银屑病严重程度的相关性，探讨IL-10的表达对银屑病的影响。方法：收集2018年10月至2019年12月温州医科大学附属第二医院育英儿童医院收治的40例银屑病患者病损皮肤，同时通过咪喹莫特（IMQ）分别诱导C57BL/6（WT）小鼠和IL-10敲除（IL-10-/-）小鼠致银屑病模型。采用PASI评分观察皮损动态变化；HE染色观察皮损组织形态学变化、表皮厚度及炎症细胞浸润程度；免疫组化检测皮损组织中IL-10和CD19蛋白表达；免疫荧光染色分析皮损组织中CD4定位改变情况。结果：IMQ造模可以取得与临床银屑病患者相类似的皮肤表现。IL-10-/-造模组小鼠背部皮损处红斑评分、鳞屑评分、皮肤浸润厚度评分及PASI总评分和耳朵厚度较WT造模组和IL-10-/-对照组均明显升高（P＜0.05）。免疫组化结果显示WT造模组小鼠皮损组织中IL-10的表达阳性率低于WT对照组，差异有统计学意义（P＜0.05）。IL-10-/-造模组小鼠CD19阳性表达率略高于IL-10-/-对照组和WT造模组，差异均有统计学意义（P＜0.05）。免疫荧光结果显示，WT造模组小鼠CD4阳性表达从表皮-真皮交界处向整个表皮层扩散，与银屑病患者样本CD4阳性定位类似。IL-10-/-造模组小鼠表皮层和真皮层均存在CD4阳性表达。结论：IL-10的缺乏会导致持续的免疫激活，加重银屑病的发生和进展，其可能通过增加鳞屑发生，促进皮肤表皮层角质形成细胞的增殖与分化，从而加重银屑病样皮损改变。

Expression characteristics of interleukin-10 and its impact on psoriasis

Objective: To analyze the characteristics of interleukin-10 (IL-10) in the skin lesions of psoriasis patients and psoriasis mouse models and its correlation with the severity of psoriasis, and to explore the effect of IL-10 expression on psoriasis. Methods: Samples of the skin lesions with psoriasis were collected from 40 patient between October 2018 and December 2019 in the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, while psoriasis-causing models were induced by imiquimod (IMQ) in C57BL/6 (WT) mice and IL-10 knockout (IL-10-/-) mice. PASI score was used to observe the dynamic changes of skin lesions. Morphological changes in tissues, epidermal thickness and degree of inflammatory cell infiltration were observed by HE staining. Immunohistochemical staining to analyze protein expressions of IL-10 and CD19 in skin lesions. Immunofluorescence staining was used to analyze the changes of CD4 localization in skin lesions. Results: IMQ model could have skin manifestations similar to clinical psoriasis. The erythema score, scale score, skin infiltration thickness score, PASI total score and ear thickness of mice were significantly higher in the IL-10-/- model group compared with WT modeling group and IL-10-/- control group (P<0.05). Immunohistochemical results showed that the positive expression rate of IL-10 in skin lesions of the WT model group was lower than that of the WT control group, with significant difference (P<0.05). The positive expression rate of CD19 in mice in IL-10-/- model group was slightly higher than that in IL-10-/- control group and WT model group, with significant difference (P<0.05). Immunofluorescence results showed that CD4 positive expression in WT model mice spread from the epidermal-dermal junction to the whole epidermis, similar to the CD4 positive localization in psoriasis patient samples. CD4 positive expression was present in both epidermis and dermis of IL-10-/- model mice. Conclusion: IL-10 deficiency leads to sustained immune activation and exacerbates the onset and progression of psoriasis, which may aggravate psoriasis-like lesion changes by increasing scaling occurrence and promoting the proliferation and differentiation of keratin-forming cells in the epidermal layer of the skin.