lncRNA H19在心力衰竭中的临床意义及对心肌细胞凋亡的影响

目的 探讨lncRNA H19(H19)在心力衰竭(heart failure, HF)中的临床意义以及通过转化生长因子-β(transforming growth factor-β,TGF-β)/重组人Smad3蛋白(SMAD family member 3,Smad3)信号通路对心肌细胞的影响机制。方法 选择2018年5月至2021年5月收治的327例HF患者(研究组)以及50例性别与年龄匹配的单纯糖尿病(或高血压)患者(对照组),分析H19在HF中的表达以及其临床意义。分离1～2日龄Sprague-Dawley大鼠心室肌细胞，构建H19低表达载体(si-H19)和TGF-β1过表达载体(pcDNA3.1),探究H19对心肌细胞凋亡的影响以及作用机制。结果 在HF患者中H19也出现表达水平上升，并且对HF具有一定诊断价值，其曲线下面积(area under curve, AUC)为0.874,并且H19是HF的独立危险因素。沉默H19后，TGF-β1/Smad3通路表达与心肌细胞凋亡均降低。值得注意的是，过表达TGF-β1仅部分恢复了沉默H19对心肌细胞凋亡的抑制作用。结论 H19...

Clinical significance of lncRNA H19 in heart failure and its effect on cardiomyocyte apoptosis

Objective To investigate the clinical significance of lncRNA H19 (H19) in heart failure (HF) and its effect on cardiomyocytes through the transforming growth factor-β (TGF-β)/MAD family member 3 (Smad3) signaling pathway. Methods From May 2018 to May 2021, 327 HF patients were selected as the research group, as well as 50 sex- and age-matched patients with simple diabetes (or hypertension) were enrolled as control group, to analyze the expression of H19 in HF and its clinical significance. The 1-2 days old Sprague-Dawley rat ventricular myocytes were isolated. H19 low expression vector (si-H19) and TGF-β1 overexpression vector (pcDNA3.1) were constructed to investigate the effect of H19 on cardiomyocyte apoptosis and its mechanism. Results The expression level of H19 was increased in HF patients, and had a certain diagnostic value for HF. Its area under curve (AUC) was 0.874, and H19 was an independent risk factor for HF. After silencing H19, both the expression of TGF-β1/Smad3 pathway and cardiomyocyte apoptosis were sreduced. Notably, overexpression of TGF-β1 only partially restored the inhibitory effect of silencing H19 on cardiomyocyte apoptosis. Conclusion H19 is highly expressed in HF, promotes the apoptosis of cardiomyocytes by activating the TGF-β1/Smad3 signaling pathway, and participates in the development of HF.