| 4’O-甲基补脂查尔酮B的体外抗胃癌活性 |
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| 引用本文： | 唐雅玲,易宝珠,李物兰,吴建章,张华杰. 4’O-甲基补脂查尔酮B的体外抗胃癌活性[J]. 温州医科大学学报, 2023, 53(3): 189-194. DOI: 10.3969/j.issn.2095-9400.2023.03.003 |
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| 作者姓名： | 唐雅玲 易宝珠 李物兰 吴建章 张华杰 |
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| 作者单位： | 1.温州医科大学 药学院，浙江 温州 325035；2.温州医科大学附属第二医院育英儿童医院 妇产科，浙江 温州 325027；3.温州医科大学 第一临床医学院（信息与工程学院），浙江 温州 325035；4.瓯江实验室（再生调控与眼脑健康浙江省实验室），浙江 温州 325101 |
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| 基金项目： | 浙江省自然科学基金项目（LGF20B020001）。 |
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| 摘 要： | 目的：研究4’O-甲基补脂查尔酮B的体外抗胃癌活性。方法：4’O-甲基补脂查尔酮B孵育胃癌细胞系AGS后，采用MTT和集落克隆形成实验检测细胞生长，划痕实验检测细胞迁移，流式细胞实验检测细胞周期和凋亡，Western blot实验检测双特异性酪氨酸磷酸化调节激酶1A（DYRK1A）蛋白表达。结果：4’O-甲基补脂查尔酮B对胃癌细胞AGS的IC50为（7.7±1.3）μmol/L，并能浓度依赖性地抑制集落克隆形成以及抑制迁移，诱导周期阻滞及凋亡，并抑制DYRK1A的表达（P ＜0.05）。结论：4’-O-甲基补骨脂查尔酮B能够通过抑制DYRK1A产生显著的体外抗胃癌活性。
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| 关 键 词： | 4&rsquo -O-甲基补骨脂查尔酮B 胃癌 双特异性酪氨酸磷酸化调节激酶1A |
| 收稿时间： | 2022-12-17 |
Anti-gastric cancer activity of 4’-O-methylbroussochalcone B in vitro |
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| TANG Yaling,YI Baozhu,LI Wulan,WU Jianzhang,ZHANG Huajie. Anti-gastric cancer activity of 4’-O-methylbroussochalcone B in vitro[J]. JOURNAL OF WENZHOU MEDICAL UNIVERSITY, 2023, 53(3): 189-194. DOI: 10.3969/j.issn.2095-9400.2023.03.003 |
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| Authors: | TANG Yaling YI Baozhu LI Wulan WU Jianzhang ZHANG Huajie |
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| Affiliation: | 1.School of Pharmaceutical Sciences, Wenzhou Medical University,Wenzhou 325035, China; 2.Department of Gynecology and Obstetrics, the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou 325027, China; 3.The First Clinical School of Medicine (School of Information and Engineering), Wenzhou Medical University, Wenzhou 325035, China;4.Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), Wenzhou 325101,China |
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| Abstract: | Objective: To investigate the anti-gastric cancer activity of 4’-O-methylbroussochalcone B in vitro. Methods: After the gastric cancer cell line AGS was treated with 4’-O-methylbroussochalcone B, the cell growth was detected by MTT and colony assay, cell migration by wounding healing assay, cell cycle and apoptosis by the flow cytometry, the expression of DYRK1A protein by Western blot. Results: The IC50 of 4’-Omethylbroussochalcone B on AGS was (7.7±1.3) μmol/L, which could inhibit colony formation and migration,induce cycle arrest and apoptosis, and down-regulate the expression of DYRK1A in a concentration-dependent manner (P<0.05). Conclusion: 4’-O-methylbroussochalcone B has significant anti-gastric cancer activity by inhibiting DYRK1A in vitro. |
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| Keywords: | 4’-O-methylbroussochalcone B gastric cancer DYRK1A |
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