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| 视网膜色素上皮细胞蛋白质组学研究中二维电泳条件的优化 | |
| 引用本文： | 李颖,邢怡桥,喻红,陈长征,侯炜,杜磊,李猛,魏礼清. 视网膜色素上皮细胞蛋白质组学研究中二维电泳条件的优化[J]. 武汉大学学报(医学版), 2007, 28(3): 279-281,291 |
| 作者姓名： | 李颖 邢怡桥 喻红 陈长征 侯炜 杜磊 李猛 魏礼清 |
| 作者单位： | 1. 武汉大学人民医院眼科/武汉大学医学院眼科研究所,湖北,武汉,430060 2. 武汉大学医学院生物化学与分子生物学系,湖北,武汉,430071 3. 武汉大学医学院病毒学国家重点实验室/病毒学研究所,湖北,武汉,430071 |
| 基金项目： | 国家自然科学基金 , 武汉市科技局攻关计划基金 |
| 摘 要： | 目的:优化并确立一套适合视网膜色素上皮细胞二维电泳的实验方法。方法:提取视网膜色素上皮细胞可溶性蛋白,改进条件以确立对视网膜色素上皮细胞蛋白进行二维电泳的实验流程。结果:该实验条件对视网膜色素上皮细胞蛋白达到了良好的分离效果,并能通过分析找出正常与病变细胞蛋白表达差异。结论:该优化的实验流程能有效分离视网膜色素上皮细胞蛋白质,并为进一步的差异蛋白质组研究奠定基础。 |
| 关 键 词： | 视网膜色素上皮细胞 二维电泳 蛋白质组 |
| 文章编号： | 1671-8852（2007）03-0279-03 |
| 修稿时间： | 2007-01-22 |
Optimized Two-dimensional Electrophoresis for Proteomic Analysis of Retinal Pigment Epithelium Cells |
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| LI Ying,XING Yiqiao,YU Hong,CHEN Changzheng,HOU Wei,DU Lei,LI Meng,WEI Liqing. Optimized Two-dimensional Electrophoresis for Proteomic Analysis of Retinal Pigment Epithelium Cells[J]. Medical Journal of Wuhan University, 2007, 28(3): 279-281,291 | |
| Authors: | LI Ying XING Yiqiao YU Hong CHEN Changzheng HOU Wei DU Lei LI Meng WEI Liqing |
| Affiliation: | 1.Dept. of Ophthalmology ,Renmin Hospital of Wuhan University, Wuhan 430060, China; 2. Dept. of Biochemistry and Molecular Biology; 3. State Key Laboratory of Virology Institute of Virology ,School of Medicine, Wuhan University, Wuhan 430071 , China |
| Abstract: | Objective: To optimize and establish two-dimensional electrophoresis(2-DE) steps for proteome analysis of retinal pigment epithelium(RPE) cells.Methods: Total soluble proteins of ARPE-19 cells were extracted,then experimental conditions were changed to find out the best one for RPE.Results: There were 300±10 spots apparently displayed,and differential expressing proteins can be found out after image analyzing of 2-DE gels between the experiment group and the normal control group.Conclusion: These 2-DE steps meet the qualifications for research in RPE cells proteome and are useful to further study on differential proteome profiles. |
| Keywords: | Retinal Pigment Epithelium Two-dimensional Electrophoresis Proteomics |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |