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Automated synthesis of 11C‐β‐hydroxybutyrate by enzymatic conversion of 11C‐acetoacetate using β‐hydroxybutyrate dehydrogenase |
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| Authors: | Sébastien Tremblay René Ouellet François Bénard Stephen C. Cunnane |
| Affiliation: | 1. Research Center on Aging, Sherbrooke, Que., Canada;2. Department of Nuclear Medicine and Radiobiology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, Que., Canada J1H 5N4;3. Sherbrooke Molecular Imaging Centre, Etienne‐LeBel Clinical Research Centre, Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, Que., Canada;4. Department of Medicine, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, Que., Canada J1H 5N4;5. Department of Physiology and Biophysics, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, Que., Canada J1H 5N4 |
| Abstract: | 1‐[11C]‐β‐hydroxybutyrate was produced by conversion from 1‐[11C]‐acetoacetate using (D)‐β‐hydroxybutyrate dehydrogenase in the presence of nicotinamide adenine dinucleotide with purification by ion exchange column chromatography. Radiochemical yield at the end of the synthesis was 10% for a total synthesis time of 36 min. High‐performance liquid chromatography analysis showed ≤4% impurities, principally unconverted acetoacetate. Residual tetrahydrofuran (34±11 ppm) and ethanol (77±30 ppm) were well under the tolerable limits for human studies. Copyright © 2008 John Wiley & Sons, Ltd. |
| Keywords: | ketone bodies acetoacetate β ‐hydroxybutyrate β ‐hydroxybutyrate dehydrogenase PET [11C]CO2 |