Exendin-4改善氧化应激减轻t-BHP诱导的HUVECs凋亡

目的 探讨Exendin-4对第三丁基过氧化氢(t-BHP)诱导人脐静脉内皮细胞(HUVECs)凋亡的影响.方法 在原代培养的HUVECs中,采用t-BHP(100 μmol/L)干预4 h,加用或不加用Exendin-4(100 nmol/L)预处理细胞24 h,分为:对照组(A组)、t-BHP处理组(B组)、Exendin-4处理组(C组)及Exendin-4+t-BHP组(D组).应用MTT比色法检测细胞存活率,DAPI染色荧光显微镜观察细胞凋亡率,活性氧(ROS)检测试剂盒检测ROS的产生.结果 与A组比较,100 μmol/L t-BHP作用4 h,B组的HUVECs存活率下降40%(P<0.001);DAPI染色荧光显微镜观察证实,t-BHP可以诱导HUVECs凋亡;加用Exendin-4预处理细胞24 h,D组的HUVECs存活率较B组增高(P<0.01).DAPI染色荧光显微镜观察细胞凋亡率提示,与B组比较,Exendin-4预处理可使D组的细胞凋亡率减少44%(P<0.05),并减少ROS的产生(P<0.05).结论 t-BHP能够诱导HUVECs凋亡,Exendin-4可减少t-BHP诱导的HUVECs凋亡.

Exendin-4 Mitigated Oxidative Stress and Protected HumanUmbilical Vein Endothelial Cells from t-BHP-induced Apoptosis

Objective To explore the effect of Exendin-4 on human umbilical vein endothelial cells(HUVECs)apoptosis induced by tert-butyl hydroperoxide(t-BHP).Methods Primary HUVECs were induced by t-BHP(100 μmol/L) for 4 h,with or without pretreatment of Exendin-4(100 nmol/L)for 24 h.The cells were divided into four groups: control group(A group),t-BHP group(B group),Exendin-4 group(C group) and Exendin-4+t-BHP(D group).MTT assay was used to measure the cell viability,and fluorescence microscopic analysis with DAPI staining was used to evaluate the apoptotic rate of HUVECs.Reactive oxygen species(ROS) detection kit was used to detect the generation of ROS.Results Following t-BHP administration,the HUVECs viability of B group decreased about 40%(P<0.001)compared to A group and fluorescence microscopy confirmed that t-BHP was inducing HUVECs apoptosis.The viability of HUVECs of D group was increased than that of B group(P<0.01).The fluorescence microscopic analysis with DAPI staining showed that the apoptosis rate of HUVECs of D group decreased about 44% (P<0.05)than that of B group.The Generation of ROS was attenuated by Exendin-4 pretreatment compared to B group(P<0.05).Conclusion t-BHP could induce apoptosis of primary HUVECs,and Exendin-4 could protect HUVECs from t-BHP-induced apoptosis.