雷公藤内酯醇对肝癌H22细胞增殖凋亡及COX-2表达的影响

目的 观察雷公藤内酯醇(TPL)对肝癌H22细胞增殖、凋亡及环氧合酶-2(COX-2)表达水平的影响. 方法 以体外培养的鼠源性H22细胞株为研究对象,实验分对照组和不同浓度的TPL处理组.MTT比色法测定TPL对H22细胞株增殖抑制情况;TPL处理48 h后,Annexin V-FITC/PI双标记流式细胞术检测细胞凋亡情况,流式细胞术间接免疫荧光双标记法检测COX-2表达变化情况. 结果 随着药物浓度的增加及时间的延长,TPL能明显抑制H22细胞增殖.TPL诱导细胞凋亡及下调COX-2的表达具有浓度依赖性,不同浓度组比较,差别具有统计学意义(P<0.05). 结论 TPL可能通过诱导肝癌H22细胞凋亡及抑制COX-2表达发挥抗肿瘤作用.

Effects of Triptolide on Proliferation, Apoptosis, and Cycloxygenase-2 Expressionin Hepatic Cancer Cell Line H22

Objective To investigate the effects of triptolide (TPL) on proliferation, apoptosis, and cycloxygenase-2 (COX-2) expression in liver cancer line H22 and explore its anti-tumor mechanism.Methods H22 cultured in vitro was treated with different concentrations of triptolide, cell proliferation activity was determined by MTT assay.The Annexin V-FITC/PI cell apoptosis and COX-2 expression were assessed at 48 h after the onset of drug treatment by flow cytometry.Result Triptolide significantly inhibited the proliferation of H22 cells in a dose-and time-dependent manner.In addition, triptolide significantly induced cell apoptosis and down-regulated COX-2 expression in a dose-manner during 48 h.Conclusion TPL may play an anti-liver cancer role by inducing cell apoptosis and inhibiting COX-2 expression in H22 cells.