夏枯草提取物抑制大鼠肾草酸钙结石形成作用的研究

目的 研究夏枯草提取物对大鼠肾草酸钙结石的作用及机制. 方法 将40只雄性Wistar大鼠随机分为4组:对照组、模型组、夏枯草提取物低剂量(50 g/L)和高剂量(100 g/L)实验组.实验4周后处死动物,将各组大鼠肾脏标本制成组织切片,H-E染色,观察大鼠肾组织病理学改变;检测大鼠血清尿素氮(BUN)、血清肌酐(Cr)、磷(P)和Ca2+的含量,检测24 h尿液中Ca2+和草酸(Ox)的含量;运用荧光定量PCR和Western-blot检测大鼠肾内TRPV5 mRNA和蛋白的表达,并进行统计学分析. 结果 与对照组比较,模型组大鼠肾呈现草酸钙结石病理改变,不同浓度的夏枯草提取物作用后,可明显改善其病理变化.模型组血清BUN,Cr,Ca2+和P含量明显升高,经不同浓度的夏枯草提取物作用后,实验组BUN及Cr含量明显低于模型组(P＜0.05),但实验组和模型组Ca2+及P含量比较无显著性差异;模型组尿液Ca2+,Ox含量较对照组明显升高,经不同浓度的夏枯草提取物作用后,可降低实验组Ca2+含量,且成药物剂量依赖性(P＜0.05),但对Ox的含量无显著性作用;模型组肾内TRPV5mRNA和蛋白的表达明显降低,经不同浓度的夏枯草水提取物作用后,实验组TRPV5 mRNA和蛋白的表达逆转,且具有统计学意义(P＜0.05). 结论 夏枯草对大鼠肾草酸钙结石具有防治作用;可能是通过上调大鼠肾脏尿钙调控信号TRPV5的表达,降低血清中BUN、Cr含量及尿液中Ca2+的含量.

The Effect and Its Mechanism of Prunella Vulgarison Inhibiting Renal Calcium Oxalate Stone Formation

Objective To study the effect of prunella vulgaris on calcium ion channel TRPV5 in rat,and investigate its mechanism on calcium oxalate nephrolithiasis.Methods The Wistar rats were randomly divided into four groups(n =10 each):A group (normal control),B group (stone formation model),C group (treated by 50 g/L methanolic extract from prunella vulgaris),D group(treated by 100 g/L methanolic extract from prunella vulgaris).The model rats with renal calcium oxalate stone formation were induced by intragastric administration of ethylene glycol and ammonium chloride.After 4 weeks,the concentration of serum creatinine(Cr),blood urea nitrogen(BUN),Ca2+,P,24 h urinary excretion Ca2+,Mg2+,and oxalate were detected.Renal samples were collected to prepare tissue slices and observe the pathological changes.The expression of TRPV5 was detected by Western blotting and real-time PCR.Results Compared with control group,nephrolithiasis rats in B group (model group) showed significant pathological changes in calcium oxalate calculus.After prunella vulgaris treatments,the renal pathological changes were shown.The concentration of Cr,BUN and urine calcium in group C and D were lower than those in group B(P＜0.05),There was no difference in serum Ca2+,P,urine Mg2+,and oxalate between group C,D and group B(P＞0.05).The expressions of TRPV5 protein and mRNA in group C and D were higher than those in group B(P＜0.05).Conclusion The results showed that prunella vulgaris may increase the expression of TRPV5 in rats,while decrease the urine Ca2+,and reduce calcium oxalate saturation.It can inhibit renal calcium oxalate stone formation.